Xu Ruiping, Zhou Shasha, Song Jiaxin, Zhong Haiying, Zhu Tianwen, Gong Yuhua, Zhou Yan, Bian Yinbing
Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Front Microbiol. 2022 Jun 20;13:910255. doi: 10.3389/fmicb.2022.910255. eCollection 2022.
As the widest cultivated edible mushroom worldwide, suffers serious yield and quality losses from heat stress during growth and development, and in our previous study, exogenous 2,4-Dichlorophenoxyacetic acid (2,4-D) was found to improve the thermotolerance of strain YS3357, but the molecular mechanism remains unclear. Here, we explored the potential protective mechanism of exogenous 2,4-D against heat stress by transcriptome analysis. 2,4-D possible improve the thermotolerance of through regulating antioxidant genes, transcription factors, energy-provision system, membrane fluidity, and cell wall remodeling. Furthermore, 2,4-D was also found to regulate the saturation levels of fatty acids and ATP content in mycelium under heat stress. This study proposed a regulatory network of 2,4-D in regulating response to heat stress, providing a theoretical basis for improving thermotolerance, and facilitating the understanding of the molecular mechanism of exogenous hormones in alleviating abiotic stress damage to macrofungi.
作为全球种植最广泛的食用蘑菇，在生长发育过程中因热胁迫遭受严重的产量和品质损失，并且在我们之前的研究中，发现外源2,4-二氯苯氧乙酸（2,4-D）可提高菌株YS3357的耐热性，但其分子机制仍不清楚。在此，我们通过转录组分析探索了外源2,4-D对抗热胁迫的潜在保护机制。2,4-D可能通过调节抗氧化基因、转录因子、能量供应系统、膜流动性和细胞壁重塑来提高其耐热性。此外，还发现2,4-D可调节热胁迫下菌丝体中脂肪酸的饱和水平和ATP含量。本研究提出了2,4-D调节应对热胁迫的调控网络，为提高其耐热性提供了理论依据，并有助于理解外源激素缓解大型真菌非生物胁迫损伤的分子机制。
