El-Dakhly Khaled Mohamed, Hany Shaimaa Ahmed, Arafa Waleed M, Abdel-Fatah Omima Ramadan, Abdel-Atty Nasser Sayed, El-Nahass El-Shaymaa
Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511 Egypt.
Fayoum University Hospitals, El Fayoum, 63522 Egypt.
J Parasit Dis. 2023 Sep;47(3):527-534. doi: 10.1007/s12639-023-01596-5. Epub 2023 May 18.
is the metacestode of the commonly defined foodborne cestode, . It infects heart, masseter muscles and other muscular sites of cattle causing bovine cysticercosis. So, a retrospective study using archival documents from authorized agencies is done during the period of 2018-2020 with estimating economic losses. Cysts were collected from municipal Beni-Suef and Basatin abattoirs. Molecular approach including cPCR and sequencing analysis is used to prove the species. The prevalence was 0.34% in Beni-Suef abattoir and 5.70% in Basatin abattoir including heads, hearts or all carcasses condemned. Among 27 provinces surveyed, the highest prevalence was recorded in Aswan followed by Cairo, Suez and Red Sea. Moreover, 19 provinces (Cairo, Alexandria, Suez, Dakahlia, Sharkia, Qalyobia, Kafrelsheikh, Gharbia, Menofiya, Ismailia, Giza, Beni-Suef, Assiut, Sohag, Qena, Aswan, Red Sea, South Sinai and El-Wadi El-Gadid) recorded bovine cysticercosis in 2018, 2019 and 2020. On the other hand, Luxor and North Sinai had no infections. The percentages of condemnation among slaughtered cattle were 1.38, 1.49 and 0.87% in 2018, 2019 and 2020, respectively. Condemnations significantly varied among north, middle and south districts of Egypt, and also varied annually. Molecularly, a diagnostic band at an amplicon size 253 bp targeting the COI gene specific for was revealed. The obtained sequences showed 100% identity with the different COI GB sequence isolates in many countries worldwide. The phylogenetic analysis method showed that the obtained sequences originating from the same clade of GB isolates globally. Careful meat inspection as well as strict hygienic measures is recommended for both veterinarians and public.
它是常见食源性绦虫的中绦期，感染牛的心脏、咬肌和其他肌肉部位，导致牛囊尾蚴病。因此，在2018 - 2020年期间利用授权机构的档案文件进行了一项回顾性研究，以估算经济损失。囊肿取自贝尼苏韦夫市和巴萨廷屠宰场。采用包括套式PCR和测序分析在内的分子方法来鉴定物种。贝尼苏韦夫屠宰场的患病率为0.34%，巴萨廷屠宰场为5.70%，包括头部、心脏或所有被判定不合格的胴体。在接受调查的27个省份中，阿斯旺的患病率最高，其次是开罗、苏伊士和红海。此外，19个省份（开罗、亚历山大、苏伊士、达卡利亚、沙尔基亚、盖勒尤比、卡夫尔谢赫、加比亚、米努菲亚、伊斯梅利亚、吉萨、贝尼苏韦夫、阿斯尤特、索哈杰、基纳、阿斯旺、红海、南西奈和新河谷）在2018年、2019年和2020年记录到牛囊尾蚴病。另一方面，卢克索和北西奈没有感染病例。2018年、2019年和2020年屠宰牛中被判定不合格的比例分别为1.38%、1.49%和0.87%。埃及北部、中部和南部地区的判定情况差异显著，且每年都有所不同。在分子水平上，针对该虫特异性COI基因的扩增子大小为253 bp处出现了一条诊断带。获得的序列与全球许多国家不同的该虫COI基因库序列分离株显示出100%的同一性。系统发育分析方法表明，获得的序列源自全球该虫基因库分离株的同一进化枝。建议兽医和公众都要进行仔细的肉类检查以及采取严格的卫生措施。
