School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru.
Laboratory of Infectious Diseases, Universidad Peruana Cayetano Heredia, Lima, Peru.
Am J Trop Med Hyg. 2018 Nov;99(5):1198-1200. doi: 10.4269/ajtmh.18-0355.
The aim of this study was to demonstrate the presence of eggs in beetles collected from sources within the natural environment through molecular techniques. Fifty-four pools of beetles were collected in three villages in Piura, Peru. DNA was extracted using the FastDNA spin kit for soil. Molecular identification of species was then performed through partial amplification of the mitochondrial cytochrome C oxidase subunit I gene. Finally, positive samples were sequenced to determine the tapeworm species. Seven positive samples were obtained through polymerase chain reaction amplification. Sequencing confirmed that two samples were from and three samples were from . The other two samples could not be specifically identified. Our findings demonstrate that dung beetles ingest and eggs under natural conditions and suggest that beetles may play a role in the dynamics of transmission of these cestodes.
本研究旨在通过分子技术证明从自然环境来源采集的甲虫中存在虫卵。在秘鲁皮乌拉的三个村庄收集了 54 个甲虫池。使用 FastDNA 土壤旋转试剂盒提取 DNA。然后通过对线粒体细胞色素 C 氧化酶亚基 I 基因的部分扩增来进行种的分子鉴定。最后,对阳性样本进行测序以确定绦虫种类。通过聚合酶链反应扩增获得了七个阳性样本。测序证实其中两个样本来自 ,三个样本来自 。另外两个样本无法进行特异性鉴定。我们的研究结果表明,在自然条件下,蜣螂会摄入 和 虫卵,并表明甲虫可能在这些绦虫的传播动态中发挥作用。