高危型人乳头瘤病毒感染对肺腺癌中 P53、pRb 和 survivin 的影响：一项回顾性研究。

Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma-a retrospective study.

机构信息

Department of Pathology, The Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong, China.

Department of Histoloembryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, China.

出版信息

PeerJ. 2023 Jul 26;11:e15570. doi: 10.7717/peerj.15570. eCollection 2023.

DOI:10.7717/peerj.15570

PMID:37520249

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10386818/

Abstract

OBJECTIVE

To observe the effects of high-risk human papillomavirus (HR-HPV) infection on P53, pRb, and survivin in lung adenocarcinoma (LUAD).

METHODS

The cancerous and cancer-adjacent tissues of 102 patients with LUAD from January 2020 to April 2022 were selected for the study. HR-HPV infection was detected by flow fluorescence method, and P53, pRb, and survivin protein expression was detected by immunohistochemical staining method. Statistical analysis was performed to determine the differences in the HR-HPV infection and the expression of P53, pRb, and survivin proteins between LUAD tissues and cancer-adjacent tissues; the correlation between HR-HPV infection and P53, pRb, and survivin protein expression in cancer tissues; and the correlation between HR-HPV infection and clinicopathological features of LUAD.

RESULTS

The infection rate of HR-HPV was higher in the LUAD tissues (28.43%) than in cancer-adjacent tissues (7.84%), and the difference was statistically significant ( < 0.05). The positive rates of P53 and survivin protein were higher in the LUAD group (33.33% and 67.16%, respectively) than in the cancer-adjacent group (3.92% and 11.73%, respectively), and the difference was statistically significant ( < 0.05). The positive rate of pRb protein was lower in the LUAD group (58.82%) than in the cancer-adjacent group (92.14%), and the difference was statistically significant ( < 0.05). The positive rates of P53 and survivin proteins were significantly higher in the HR-HPV LUAD group (58.62% and 86.21%, respectively) than in the non-HR-HPV LUAD group (41.38% and 67.12%, respectively), and the difference was statistically significant ( < 0.05). The expression rate of pRb protein was significantly lower in the HR-HPV LUAD group (37.93%) than in the non-HR-HPV LUAD group (67.12%), and the difference was statistically significant ( < 0.05). The expression of p53 and survivin protein was positively correlated with HR-HPV infection ( = 0.338 and 0.444, < 0.05), whereas the expression of pRb protein was negatively correlated with HR-HPV infection ( = - 0.268, < 0.05). HR-HPV infection was not associated with gender, age, and smoking in patients with LUAD ( > 0.05). HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD ( < 0.05).

CONCLUSIONS

HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD, which may be achieved by up-regulating p53 and survivin protein expression and down-regulating pRb protein expression.

摘要

目的

观察高危型人乳头瘤病毒（HR-HPV）感染对肺腺癌（LUAD）中 P53、pRb 和 survivin 的影响。

方法

选取 2020 年 1 月至 2022 年 4 月间 102 例 LUAD 患者的癌组织和癌旁组织进行研究。采用流式荧光法检测 HR-HPV 感染，免疫组化染色法检测 P53、pRb 和 survivin 蛋白表达。统计分析 LUAD 组织与癌旁组织中 HR-HPV 感染和 P53、pRb、survivin 蛋白表达的差异；分析癌组织中 HR-HPV 感染与 P53、pRb、survivin 蛋白表达的相关性；分析 HR-HPV 感染与 LUAD 临床病理特征的相关性。

结果

HR-HPV 感染在 LUAD 组织中的感染率（28.43%）高于癌旁组织（7.84%），差异有统计学意义（<0.05）。P53 和 survivin 蛋白在 LUAD 组中的阳性率（分别为 33.33%和 67.16%）高于癌旁组（分别为 3.92%和 11.73%），差异有统计学意义（<0.05）。pRb 蛋白在 LUAD 组中的阳性率（58.82%）低于癌旁组（92.14%），差异有统计学意义（<0.05）。HR-HPV LUAD 组中 P53 和 survivin 蛋白的阳性率（分别为 58.62%和 86.21%）明显高于非 HR-HPV LUAD 组（分别为 41.38%和 67.12%），差异有统计学意义（<0.05）。HR-HPV LUAD 组 pRb 蛋白的表达率（37.93%）明显低于非 HR-HPV LUAD 组（67.12%），差异有统计学意义（<0.05）。P53 和 survivin 蛋白的表达与 HR-HPV 感染呈正相关（=0.338 和 0.444，<0.05），而 pRb 蛋白的表达与 HR-HPV 感染呈负相关（= -0.268，<0.05）。HR-HPV 感染与 LUAD 患者的性别、年龄和吸烟状况无关（>0.05）。HR-HPV 感染与 LUAD 的淋巴结转移和临床分期有关（<0.05）。