Qi Qinbing, Li Rongkai, Wang Chunhua, Hou Guige, Li Chengbo
Key Laboratory of Medical Antibacterial Materials of Shandong Province, School of Pharmacy, Binzhou Medical University, Yantai, China.
Front Microbiol. 2023 Jul 14;14:1233559. doi: 10.3389/fmicb.2023.1233559. eCollection 2023.
The development of highly effective wound dressings is crucial for successful clinical applications. Achieving wound closure, preventing infection, and minimizing scarring are key objectives in wound healing. Drawing inspiration from the regenerative mechanisms observed in embryonic tissue repair, we designed a series of wound-contractible dressings with exceptional antibacterial properties.
This was achieved by encapsulating quaternized silicone (QP12) and poly(-isopropylacrylamide-co--hydroxymethylacrylamide-co-octadecyl acrylate) (PNNS) within electrospun nanofibers of poly(ε-caprolactone) (PCL).
The resulting nanofibrous dressings demonstrated remarkable thermo-responsive self-contraction and tissue adhesion capabilities, enabling secure adherence to the skin and active wound closure. Notably, these nanofibers exhibited potent antibacterial activity against both Gram-positive and Gram-negative bacteria. Furthermore, they possessed desirable properties such as hydrophilicity, biocompatibility and mechanical properties resembling human skin. A full-thickness skin defect model evaluation revealed that these temperature-sensitive nanofibers expedited wound closure, enhanced wound healing, and suppressed scar formation. This result was evidenced by reduced infiltration of inflammatory cells, well-organized collagen arrangement, and improved vascularization. In summary, we propose that these wound-contractible nanofibers, with their antibacterial and anti-scarring properties, hold great promise as an advanced solution for skin wound repair.
开发高效的伤口敷料对于成功的临床应用至关重要。实现伤口闭合、预防感染和最小化疤痕形成是伤口愈合的关键目标。从胚胎组织修复中观察到的再生机制中汲取灵感，我们设计了一系列具有卓越抗菌性能的可收缩伤口敷料。
通过将季铵化硅氧烷（QP12）和聚（-异丙基丙烯酰胺-co--羟甲基丙烯酰胺-co-丙烯酸十八酯）（PNNS）封装在聚（ε-己内酯）（PCL）的电纺纳米纤维中来实现。
所得的纳米纤维敷料表现出显著的热响应自收缩和组织粘附能力，能够牢固地粘附在皮肤上并实现主动伤口闭合。值得注意的是，这些纳米纤维对革兰氏阳性菌和革兰氏阴性菌均表现出强大的抗菌活性。此外，它们具有诸如亲水性、生物相容性和类似于人类皮肤的机械性能等理想特性。全层皮肤缺损模型评估表明，这些温度敏感的纳米纤维加速了伤口闭合，促进了伤口愈合，并抑制了疤痕形成。炎症细胞浸润减少、胶原蛋白排列有序以及血管生成改善证明了这一结果。总之，我们认为这些具有抗菌和抗疤痕特性的可收缩伤口纳米纤维作为皮肤伤口修复的先进解决方案具有巨大潜力。
