Li Rongkai, Qi Qinbing, Jiang Xiaojing, Gao Zhongfei, Xie Xianrui, Zhao Yuqing, Cheng Xiaochen, Wang Chunhua, Hou Guige, Li Chengbo
School of Pharmacy, Key Laboratory of Medical Antibacterial Materials of Shandong Province, Binzhou Medical University, Yantai, China.
Biomedical Laboratory, School of Medicine, Liaocheng University, Liaocheng, China.
Front Microbiol. 2025 Jun 25;16:1550276. doi: 10.3389/fmicb.2025.1550276. eCollection 2025.
Infectious scald is recognized as a life-threatening concern in clinical due to easy infection and hard recovery. Exosomes possess cell migration, proliferation and angiogenesis under the containing rich growths factors, which may be a possibility for the treatment of scald chronic wounds. However, the instability and release issues of exosomes limits the storage and therapeutic efficacy. Herein, in this study, exosomes were successfully encapsulated calcium alginate microspheres, effectively avoiding the leakage and inactivation of exosomes. Meanwhile, composite hydrogel (HS-QAF-AgNPs) was fabricated with and quaternized carboxymethyl chitosan (Q-CMC), aldehyde group-modified alginate (ASA) with assisted crosslinking of ferric ion and dopped nano silver (AgNPs). The incorporation of exo-CAMs into the hydrogel protects the exosomes from harsh environmental conditions and extends their diffusion time, thereby guaranteeing a better therapeutic effect. Q-CMC and AgNPs altogether endow the hydrogel system with an inherent antibacterial effect against pathogenic microorganisms. and investigations demonstrated that the HS-QAF-AgNPs hydrogel was cytocompatibility, possessed antibacterial and hemostatic properties, promoted cell migration and cell proliferation, stimulated angiogenesis, regulated inflammatory response, delayed apoptosis, enhanced collagen deposition and accelerated wound closure. Thus, the multifunctional HS-QAF-AgNPs hydrogel dressing is expected to be a promising candidate for the treatment of severe scalds. There is an urgent need to develop multifunctional wound dressings that can coordinate cell migration, proliferation, apoptosis, collagen formation and remodeling, alongside inflammation and angiogenesis to promote skin wound closure.
感染性烫伤在临床上被认为是一个危及生命的问题，因为其容易感染且难以恢复。外泌体在含有丰富生长因子的情况下具有细胞迁移、增殖和血管生成能力，这可能为治疗烫伤慢性伤口提供一种可能性。然而，外泌体的不稳定性和释放问题限制了其储存和治疗效果。在此，在本研究中，外泌体成功地被包裹在海藻酸钙微球中，有效避免了外泌体的泄漏和失活。同时，用季铵化羧甲基壳聚糖（Q-CMC）、醛基修饰的海藻酸盐（ASA）并借助铁离子辅助交联和掺杂纳米银（AgNPs）制备了复合水凝胶（HS-QAF-AgNPs）。将外泌体-钙黏蛋白（exo-CAMs）掺入水凝胶中可保护外泌体免受恶劣环境条件的影响，并延长其扩散时间，从而保证更好的治疗效果。Q-CMC和AgNPs共同赋予水凝胶系统对致病微生物的固有抗菌作用。 研究表明，HS-QAF-AgNPs水凝胶具有细胞相容性，具有抗菌和止血性能，促进细胞迁移和细胞增殖，刺激血管生成，调节炎症反应，延迟细胞凋亡，增强胶原蛋白沉积并加速伤口愈合。因此，多功能HS-QAF-AgNPs水凝胶敷料有望成为治疗严重烫伤的有前途的候选者。迫切需要开发能够协调细胞迁移、增殖、凋亡、胶原蛋白形成和重塑以及炎症和血管生成以促进皮肤伤口闭合的多功能伤口敷料。
