Manyawi Monica, Mozirandi Winnie Yevai, Tagwireyi Dexter, Mukanganyama Stanley
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Medicine and Health Sciences, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe.
Department of Biotechnology and Biochemistry, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe.
ScientificWorldJournal. 2023 Jul 21;2023:2322068. doi: 10.1155/2023/2322068. eCollection 2023.
is traditionally used for the treatment of many infections, including bacterial infections. The aim of this study was to fractionate and evaluate antibacterial activity of the crude extract of , as well as the surface compounds from the leaves of , in two pathogenic bacteria, and . The antibacterial activities of fractions obtained from chromatographic separations were determined using broth microdilution assay on the laboratory and clinical strains of and . The fractionation of the compounds on the leaf surface yielded 262 fractions. The fractionated compounds with similar TLC profiles were pooled together to yield 47 pools. The extract and pooled fractions CZSC151154, CZSC155160, and CZSC209213 showed significant antibacterial activity with MIC values ranging from 12.5 g/ml to 100 g/ml. The clinical strain of had MIC greater than 100 g/ml for CZSC151154 and CZSC155160. The minimum bactericidal concentration values for these fractions were also in the range of 12.5 g/ml to 100 g/ml. The extract and fractions CZSC151154, CZSC155160, and CZSC209213 showed a concentration-dependent inhibition of growth in . Analyses of the CZSC209213 pool by LC-MS showed the presence of nine compounds which are (3R,7R)-1,3,7-octanetriol, (-)-tortuosamine, 11-aminoundecanoic acid, 1-piperidinecarboxaldehyde, 3-hydroxy-4-isopropylbenzyl alcohol 3-glucoside, hydroxy-isocaproic acid, oleamide, palmitic amide, phytospingosine, and sphinganine. In conclusion, leaf surface compounds exhibited antibacterial activity. The crude extract and the pooled fractions showed concentration-dependent inhibition of growth on Results from this study indicate the potential of as a source of lead compounds that may be further developed into antibacterial drugs.
传统上用于治疗包括细菌感染在内的多种感染。本研究的目的是分离并评估[植物名称]粗提物以及[植物名称]叶片表面化合物对两种病原菌[病原菌名称1]和[病原菌名称2]的抗菌活性。使用肉汤微量稀释法对[病原菌名称1]和[病原菌名称2]的实验室菌株和临床菌株测定色谱分离得到的各馏分的抗菌活性。叶片表面化合物的分离得到了262个馏分。具有相似薄层色谱图谱的分离化合物合并在一起得到47个合并物。提取物以及合并物CZSC151154、CZSC155160和CZSC209213表现出显著的抗菌活性，最低抑菌浓度值范围为12.5μg/ml至100μg/ml。对于CZSC151154和CZSC155160，[病原菌名称1]的临床菌株最低抑菌浓度大于100μg/ml。这些馏分的最低杀菌浓度值也在12.5μg/ml至100μg/ml范围内。提取物以及馏分CZSC151154、CZSC155160和CZSC209213对[病原菌名称1]的生长表现出浓度依赖性抑制。通过液相色谱 - 质谱联用对CZSC209213合并物进行分析，结果显示存在9种化合物，分别为(3R,7R)-1,3,7 - 辛三醇、(-)-tortuosamine、11 - 氨基十一烷酸、1 - 哌啶甲醛、3 - 羟基 - 4 - 异丙基苄醇3 - 葡萄糖苷、羟基异己酸、油酰胺、棕榈酰胺、植物鞘氨醇和鞘氨醇。总之，[植物名称]叶片表面化合物表现出抗菌活性。粗提物和合并馏分对[病原菌名称1]的生长表现出浓度依赖性抑制。本研究结果表明[植物名称]作为先导化合物来源的潜力，这些先导化合物可能进一步开发成抗菌药物。
