Antiparasitic Activity of Isolated Fractions from Kunth against the Hemoflagellate Protozoan .

This study focused on isolating, identifying, and evaluating the trypanocidal potential against the hemoflagellate protozoan of compounds from (Mariola), a plant used in traditional Mexican medicine to treat stomach and liver disorders. has a wide distribution in Mexico. This study found that methanolic extracts of , obtained by static maceration and successive reflux, had promising results. The fractions were compared using thin-layer chromatography (TLC) and those that showed similarities were mixed. A bioguided assay was performed with ATCC 25923, using agar diffusion and bioautography techniques to determine the preliminary biological activity. The fractions with antimicrobial activity were purified using a preparative thin-layer chromatography (PTLC) plate, obtaining the bioactive bandages that were subjected to a trypanocidal evaluation against the Ninoa strain of in its epimastigote stage. This revealed an IC of up to 45 ± 2.5 µg/mL, in contrast to the values obtained from the crude extracts of less than 100 µg/mL. The TLC, Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) techniques were used to identify the compounds, demonstrating the presence of sesquiterpene lactones, parthenin, and coronopolin. We concluded that these compounds have the potential to inhibit growth.