Hernández-Marín David Alejandro, Castro-Rios Rocio, Chávez-Montes Abelardo, Castillo-Hernández Sandra L, Elizondo-Luevano Joel Horacio, Muñoz-Ortega Martín Humberto, Sánchez-García Eduardo
Departamento de Microbiología, Centro de Ciencias Básicas, Benemérita Universidad Autónoma de Aguascalientes, Aguascalientes 20100, AG, Mexico.
Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey 66460, NL, Mexico.
Antibiotics (Basel). 2024 Jul 4;13(7):622. doi: 10.3390/antibiotics13070622.
This study focused on isolating, identifying, and evaluating the trypanocidal potential against the hemoflagellate protozoan of compounds from (Mariola), a plant used in traditional Mexican medicine to treat stomach and liver disorders. has a wide distribution in Mexico. This study found that methanolic extracts of , obtained by static maceration and successive reflux, had promising results. The fractions were compared using thin-layer chromatography (TLC) and those that showed similarities were mixed. A bioguided assay was performed with ATCC 25923, using agar diffusion and bioautography techniques to determine the preliminary biological activity. The fractions with antimicrobial activity were purified using a preparative thin-layer chromatography (PTLC) plate, obtaining the bioactive bandages that were subjected to a trypanocidal evaluation against the Ninoa strain of in its epimastigote stage. This revealed an IC of up to 45 ± 2.5 µg/mL, in contrast to the values obtained from the crude extracts of less than 100 µg/mL. The TLC, Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) techniques were used to identify the compounds, demonstrating the presence of sesquiterpene lactones, parthenin, and coronopolin. We concluded that these compounds have the potential to inhibit growth.
本研究着重于分离、鉴定和评估从墨西哥传统医学中用于治疗胃部和肝脏疾病的植物（马里奥拉）中提取的化合物对血液鞭毛虫原生动物的杀锥虫潜力。该植物在墨西哥分布广泛。本研究发现，通过静态浸渍和连续回流获得的该植物甲醇提取物有良好结果。使用薄层色谱法（TLC）对各馏分进行比较，并将显示相似性的馏分混合。采用琼脂扩散和生物自显影技术，对ATCC 25923进行生物导向测定，以确定初步生物活性。使用制备型薄层色谱（PTLC）板对具有抗菌活性的馏分进行纯化，得到生物活性绷带，对处于前鞭毛体阶段的克氏锥虫尼诺阿菌株进行杀锥虫评估。结果显示，其半数抑制浓度（IC）高达45±2.5微克/毫升，相比之下，粗提物的值小于100微克/毫升。运用TLC、傅里叶变换红外光谱（FT-IR）以及高效液相色谱-质谱联用（HPLC-MS）技术鉴定化合物，证明存在倍半萜内酯、蜂斗菜素和冠突毒素。我们得出结论，这些化合物具有抑制克氏锥虫生长 的潜力。
