用于翻译光学控制的芳基偶氮吡唑苯丙氨酸的遗传编码

Janosko Chasity P, Shade Olivia, Courtney Taylor M, Horst Trevor J, Liu Melinda, Khare Sagar D, Deiters Alexander

Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.

Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey 08854, United States.

ACS Omega. 2023 Jul 14;8(29):26590-26596. doi: 10.1021/acsomega.3c03512. eCollection 2023 Jul 25.

An arylazopyrazole was explored for its use as an enhanced photoswitchable amino acid in genetic code expansion. This new unnatural amino acid was successfully incorporated into proteins in both bacterial and mammalian cells. While photocontrol of translation required pulsed irradiations, complete selectivity for the -configuration by the pyrrolysyl tRNA synthetase was observed, demonstrating expression of a gene of interest selectively controlled via light exposure.

研究了一种芳基偶氮吡唑作为遗传密码扩展中增强型光开关氨基酸的用途。这种新的非天然氨基酸已成功地在细菌和哺乳动物细胞中整合到蛋白质中。虽然翻译的光控需要脉冲照射，但观察到吡咯赖氨酸tRNA合成酶对α-构型具有完全选择性，这表明通过光照可选择性地控制感兴趣基因的表达。