Department of Pharmacology, University of California, San Diego, La Jolla, CA, 92093, USA.
Department of Pharmacology, University of California, San Diego, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, USA; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093, USA.
Curr Opin Cell Biol. 2020 Apr;63:114-124. doi: 10.1016/j.ceb.2020.01.007. Epub 2020 Feb 10.
Cells rely on a complex network of spatiotemporally regulated signaling activities to effectively transduce information from extracellular cues to intracellular machinery. To probe this activity architecture, researchers have developed an extensive molecular tool kit of fluorescent biosensors and optogenetic actuators capable of monitoring and manipulating various signaling activities with high spatiotemporal precision. The goal of this review is to provide readers with an overview of basic concepts and recent advances in the development and application of genetically encodable biosensors and optogenetic tools for understanding signaling activity.
细胞依赖于时空调节的信号转导活动的复杂网络，以有效地将信息从细胞外线索传递到细胞内机制。为了探测这种活动结构，研究人员开发了广泛的荧光生物传感器和光遗传学驱动器的分子工具包，能够以高时空精度监测和操纵各种信号转导活动。本文的目的是为读者提供一个概述，介绍用于理解信号转导活性的遗传可编码生物传感器和光遗传学工具的开发和应用的基本概念和最新进展。
