Chen Ke-Jun, Han Yang, Wang Zi-Yi, Cui Yong
Department of Dermatology, China-Japan Friendship Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Department of Dermatology, China-Japan Friendship Hospital, Beijing, China.
Exp Dermatol. 2023 Oct;32(10):1613-1623. doi: 10.1111/exd.14899. Epub 2023 Jul 31.
Non-invasive optical examination plays a crucial role in various aspects of dermatology, such as diagnosis, management and research. Multiphoton microscopy uses a unique submicron technology to stimulate autofluorescence (AF), allowing for the observation of cellular structure, assessment of redox status and quantification of collagen fibres. This advanced imaging technique offers dermatologists novel insights into the skin's structure, positioning it as a promising 'stethoscope' for future development in the field. This review provides an overview of multiphoton microscopy's principles, technology and application in studying normal skin, tumour and inflammatory diseases, as well as collagen-related and pigmentary diseases.
非侵入性光学检查在皮肤科的各个方面，如诊断、管理和研究中都起着至关重要的作用。多光子显微镜使用独特的亚微米技术来激发自发荧光（AF），从而能够观察细胞结构、评估氧化还原状态并量化胶原纤维。这种先进的成像技术为皮肤科医生提供了关于皮肤结构的新见解，使其成为该领域未来发展中一个有前景的“听诊器”。本文综述了多光子显微镜的原理、技术及其在研究正常皮肤、肿瘤、炎症性疾病以及胶原相关和色素性疾病中的应用。
