Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Planta. 2023 Jul 31;258(3):56. doi: 10.1007/s00425-023-04211-2.
Taetr1-1 can promote enhanced seed dormancy and ethylene insensitivity in wheat, indicating a conserved function of ETR1 in regulating seed dormancy. Lots of wheat cultivars have weak dormant seed. Weak seed dormancy can cause pre-harvest sprouting (PHS) in grain which significantly reduces grain yield and quality. The mining of causal genes of PHS resistance will serve to enhance breeding selection and cultivar development. In a previous study in Arabidopsis, we identified reduced dormancy 3 as a loss-of-function mutant of the ethylene receptor 1 (ETR1), which can control seed dormancy through the ERF12-TPL-DOG1 pathway. However, it is unknown whether ETR1 also functions in the regulation of wheat seed dormancy. To identify the regulatory role of ETR1 in wheat, we cloned TaETR1 and overexpressed the gain-of-function mutant Taetr1-1. The result indicated that overexpression of Taetr1-1 can promote enhanced seed dormancy and ethylene insensitivity in wheat. This study contributed to our understanding of the molecular basis for the regulation of wheat PHS resistance.
Taetr1-1 可以促进小麦增强的种子休眠和乙烯不敏感性，表明 ETR1 在调节种子休眠方面具有保守功能。许多小麦品种的种子休眠能力较弱。弱种子休眠会导致收获前发芽（PHS），从而显著降低谷物产量和质量。对 PHS 抗性相关基因的挖掘有助于增强品种选育和开发。在之前的拟南芥研究中，我们发现休眠减少 3 是乙烯受体 1（ETR1）的功能丧失突变体，它可以通过 ERF12-TPL-DOG1 途径来控制种子休眠。然而，ETR1 是否也在小麦种子休眠的调节中起作用尚不清楚。为了鉴定 ETR1 在小麦中的调节作用，我们克隆了 TaETR1 并过表达了功能获得型突变体 Taetr1-1。结果表明，过表达 Taetr1-1 可以促进小麦增强的种子休眠和乙烯不敏感性。这项研究有助于我们理解小麦 PHS 抗性的分子基础。
