Laboratory of Plague, Masgut Aikimbayev National Scientific Center for Especially Dangerous Infections of the Ministry of Healthcare of the Republic of Kazakhstan, Almaty, Republic of Kazakhstan.
Department of Express Diagnostics and Indication of Especially Dangerous Infections, Masgut Aikimbayev National Scientific Center for Especially Dangerous Infections of the Ministry of Healthcare of the Republic of Kazakhstan, Almaty, Republic of Kazakhstan.
Comp Immunol Microbiol Infect Dis. 2023 Sep;100:102025. doi: 10.1016/j.cimid.2023.102025. Epub 2023 Jul 15.
The relevance of the problem of the stated topic lies in the fact that the causative agent of the plague infection demonstrates high survival while maintaining high virulence in the territories, which are enzootic in terms of the plague. The study aimed to investigate the geographic distribution and genetic diversity of the plague pathogen in endemic regions through molecular genetic research. The work included the results of laboratory studies of 3058 samples, including soil - 1154, burrow substrates - 549, the contents of the feeding chamber - 349, bone remains - 18, biological objects - 988 samples of sera and suspensions from carriers and vectors of plague infection collected from 14 autonomous plague foci of Kazakhstan for the period 2021-2022. The leading method in the study was a laboratory experiment, thanks to which, using a new advanced technology on a microbiological analyser VITEK 2 COMPACT 30, it was possible to study pathogenic and non-pathogenic strains of the genus Yersinia isolated during field experiment. As a result of experimental work, it was shown that during a long inter-epizootic period, the plague pathogen can persist in the soil in symbiosis with soil microorganisms, and in this area, it chooses soil with a low-quality index of 10 points, where soils with a low total microbial number and species landscape prevail.
该主题问题的相关性在于，鼠疫感染的病原体在鼠疫地方性流行地区具有很高的生存能力，同时保持着高毒力。本研究旨在通过分子遗传学研究调查地方性流行地区鼠疫病原体的地理分布和遗传多样性。该工作包括对 3058 个样本的实验室研究结果，其中包括土壤样本 1154 个、洞穴基质样本 549 个、取食室内容物样本 349 个、骨骼样本 18 个、生物样本 988 个，这些样本来自于 2021 年至 2022 年期间哈萨克斯坦 14 个自治鼠疫疫区的鼠疫感染载体和宿主的血清和悬浮液。研究中的主要方法是实验室实验，通过在微生物分析仪 VITEK 2 COMPACT 30 上使用新的先进技术，该方法可以研究在野外实验中分离出的致病性和非致病性耶尔森氏菌菌株。实验结果表明，在一个漫长的流行间歇期内，鼠疫病原体可以与土壤微生物共生存在于土壤中，并在这个区域中选择土壤质量指数为 10 分的土壤，其中土壤中总微生物数量和物种景观较低。
