Analytical & Testing Center, Sichuan University, Chengdu, Sichuan 610064, China.
Analytical & Testing Center, Sichuan University, Chengdu, Sichuan 610064, China.
Food Chem. 2023 Dec 15;429:136991. doi: 10.1016/j.foodchem.2023.136991. Epub 2023 Jul 24.
Acrylamide (AA) is a carcinogen mainly ingested through food and drinking water, making its accurate determination crucial for both food safety and environmental protection. Herein, we proposed a derivatization-based ratiometric surface-enhanced Raman scattering (SERS) method for the quantification of AA. High density Au NPs were anchored to the surface of Cu-TCPP MOF nanosheets (MOFNs) to form the SERS sensor. The abundant Raman "hot spots" at the nanogaps generated by the Au NPs and the internal standard (IS) signal provided by Cu-TCPP MOFNs improved the sensitivity and quantitative accuracy of the method. Following the thiol-ene click derivatization reaction between p-aminothiophenol (PATP) and AA, the Raman peak intensity ratio (I/I) was employed to quantify AA. The linear range was 0.1 nM to 10 μM, and the limit of detection (LOD) was as low as 0.08 nM. Trace amounts of AA in food and water samples were successfully determined using this method.
丙烯酰胺（AA）是一种主要通过食物和饮用水摄入的致癌物质，因此准确测定其含量对于食品安全和环境保护都至关重要。在此，我们提出了一种基于衍生化的比率型表面增强拉曼散射（SERS）方法，用于 AA 的定量分析。高密度的 Au NPs 被锚定在 Cu-TCPP MOF 纳米片（MOFNs）的表面上，形成 SERS 传感器。Au NPs 产生的丰富的 Raman“热点”和 Cu-TCPP MOFNs 提供的内标（IS）信号提高了该方法的灵敏度和定量准确性。在 PATP 与 AA 之间的巯基-烯点击衍生化反应之后，采用 Raman 峰强度比（I/I）来定量 AA。该方法的线性范围为 0.1 nM 至 10 μM，检测限（LOD）低至 0.08 nM。该方法成功地用于测定食品和水样中的痕量 AA。
