Sun Yi, Cai Jian, Mao Haiyan, Gong Liming, Chen Yin, Yan Hao, Shi Wen, Lou Xiuyu, Su Lingxuan, Wang Xingxing, Zhou Biaofeng, Pei Zhichao, Cao Yanli, Ge Qiong, Zhang Yanjun
Zhejiang Provincial Center for Disease Control and Prevention, 3399 Binsheng Road, Hangzhou, Zhejiang 310051, China.
Zhejiang Provincial Center for Disease Control and Prevention, 3399 Binsheng Road, Hangzhou, Zhejiang 310051, China.
J Clin Virol. 2023 Sep;166:105552. doi: 10.1016/j.jcv.2023.105552. Epub 2023 Jul 23.
Coxsackievirus A10 (CA10) is one of the etiological agents associated with hand, foot and mouth disease (HFMD).
We aimed to perform a retrospective analysis of the molecular epidemiological characteristics and genetic features of HFMD associated with CA10 infections in Zhejiang Province from 2017 to 2022.
Epidemiologic features were summarized. Throat swab specimens were collected and tested. The VP1 regions were sequenced for genotyping. CA10 positive samples were isolated. Whole genomes of CA10 isolations were sequenced. Nucleotide and amino acid changes were characterized. Phylogenetic trees were constructed.
The number of HFMD cases fluctuated from 2017 to 2022. Children aged below 3 years accounted for the majority (66.29%) and boys were more frequently affected than girls. Cases peaked in June. The positivity rate of HEV was 62.69%. A total of 90 strains of CA10 were isolated and 53 genomes were obtained. All CA10 in this study could be assigned to two genogroups, C (C2) and F (F1 and F3).
The clinical manifestations of HFMD associated with HEV are complex and diverse. CA10 infection may be emerging as a new and major cause of HFMD because an upward trend was observed in the proportion of CA10 cases after the use of EV71 vaccines. Different genogroups of CA10 had different geographic distribution patterns. Surveillance should be strengthened and further comprehensive studies should be continued to provide a scientific basis for HFMD prevention and control.
柯萨奇病毒A10（CA10）是手足口病（HFMD）的病原体之一。
我们旨在对2017年至2022年浙江省与CA10感染相关的手足口病的分子流行病学特征和基因特征进行回顾性分析。
总结流行病学特征。收集并检测咽拭子标本。对VP1区域进行测序以进行基因分型。分离出CA10阳性样本。对CA10分离株的全基因组进行测序。对核苷酸和氨基酸变化进行特征分析。构建系统发育树。
2017年至2022年手足口病病例数波动。3岁以下儿童占大多数（66.29%），男孩比女孩更易感染。病例在6月达到高峰。肠道病毒（HEV）阳性率为62.69%。共分离出90株CA10，获得53个基因组。本研究中的所有CA10可分为两个基因群，C（C2）和F（F1和F3）。
与HEV相关的手足口病临床表现复杂多样。由于在使用EV71疫苗后CA10病例比例呈上升趋势，CA10感染可能正成为手足口病的一个新的主要病因。不同基因群的CA10具有不同的地理分布模式。应加强监测并继续开展进一步的综合研究，为手足口病的预防和控制提供科学依据。
