"Petru Poni" Institute of Macromolecular Chemistry of Romanian Academy, Iasi, Romania.
"Grigore T. Popa" University of Medicine and Pharmacy, Iasi, Romania.
Int J Biol Macromol. 2023 Sep 30;249:126056. doi: 10.1016/j.ijbiomac.2023.126056. Epub 2023 Jul 29.
The paper aimed to prepare quaternary chitosan-based nanofibers as bioabsorbable wound dressings. To this aim, fully biodegradable chitosan/N,N,N-trimethyl chitosan (TMC) nanofibers were designed and prepared via electrospinning, using poly(ethylene glycol) as sacrificial additive. The new biomaterials were structurally and morphologically characterized by FTIR and NMR spectroscopy, thermogravimetric analysis, X-ray diffraction and scanning electron microscopy, and their properties required for wound dressings application were investigated and discussed in detail. Thus, the nanofiber behavior was investigated by swelling, dynamic vapor sorption, and in vitro biodegradation in media mimicking the wound exudate. The mechanical properties were analysed from the stress-strain curves, the bioadhesivity from the texture analysis and the mucoadhesivity from the Zeta potential and transmittance measurements. The antimicrobial activity was assessed against S. aureus and E. coli strains, and the biocompatibility was tested in vitro on normal human dermal fibroblasts, and in vivo on rats. The application of the fiber mats with the best balance of properties as dressings on deep burn wound models in rats showed wound closure and active healing, with fully restoration of epithelia. It was concluded that the combination of chitosan with TMC into nanofibers provides new potential bioabsorbable wound dressing, opening new perspectives in regenerative medicine.
本文旨在制备季铵化壳聚糖基纳米纤维作为可生物吸收的伤口敷料。为此，通过静电纺丝设计并制备了完全可生物降解的壳聚糖/N,N,N-三甲基壳聚糖（TMC）纳米纤维，使用聚乙二醇作为牺牲添加剂。通过傅里叶变换红外光谱（FTIR）和核磁共振光谱（NMR）、热重分析（TGA）、X 射线衍射（XRD）和扫描电子显微镜（SEM）对新的生物材料进行了结构和形态表征，并详细研究和讨论了其作为伤口敷料应用所需的性能。因此，通过溶胀、动态蒸汽吸附和在模拟伤口渗出液的介质中的体外生物降解来研究纳米纤维的行为。从应力-应变曲线分析力学性能，从纹理分析分析生物附着性，从 Zeta 电位和透光率测量分析粘膜附着性。评估了对金黄色葡萄球菌和大肠杆菌菌株的抗菌活性，并在体外正常人类真皮成纤维细胞和体内大鼠上测试了生物相容性。将性能平衡最佳的纤维垫用作大鼠深度烧伤模型的敷料，显示出伤口闭合和主动愈合，上皮完全恢复。研究结果表明，壳聚糖与 TMC 结合形成纳米纤维为可生物吸收的伤口敷料提供了新的潜力，为再生医学开辟了新的前景。
