Jabraili Masoud, Moniri-Javadhesari Solmaz, Pouladi Nasser, Hosseinpour-Feizi Mohammad Ali
Department of Cellular and Molecular Biology, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Mol Biol Res Commun. 2023;12(3):127-131. doi: 10.22099/mbrc.2023.47622.1839.
Thyroid cancer is the most common malignancy of the endocrine system. LncRNAs play critical role in various cellular processes and are associated with several diseases. CCAT2 is a lncRNA molecule overexpressed in thyroid cancer. Single nucleotide polymorphisms in gene can cause changes in the structure and function of CCAT2 transcripts and susceptibility to several diseases. This study aimed to evaluate the association of rs6983267 in gene with thyroid cancer susceptibility in the Azeri population of Iran. In this "case-control" study, genomic DNA was extracted from peripheral blood of 102 individuals affected by thyroid cancer and 103 healthy individuals as controls. Genotyping was performed using TETRA-ARMS polymerase chain reaction. Statistical analysis showed no significant association between genotypes and/or alleles with the occurrence of thyroid cancer in the studied population, patients' gender, and tumor type. Nevertheless, we found that the allelic and genotypic distribution of this SNP was associated with the size of thyroid tumors in patients. It is assumed that investigating more individuals from both case and control group may further determine the genotypic and allelic frequencies of this SNP locus in Iranian-Azeri population.
甲状腺癌是内分泌系统最常见的恶性肿瘤。长链非编码RNA（lncRNAs）在各种细胞过程中发挥关键作用，并与多种疾病相关。CCAT2是一种在甲状腺癌中过表达的lncRNA分子。该基因中的单核苷酸多态性可导致CCAT2转录本的结构和功能发生变化，并影响多种疾病的易感性。本研究旨在评估该基因中rs6983267与伊朗阿塞拜疆人群甲状腺癌易感性的关联。在这项“病例对照”研究中，从102例甲状腺癌患者和103例健康对照者的外周血中提取基因组DNA。采用四引物扩增受阻突变系统聚合酶链反应进行基因分型。统计分析表明，在研究人群、患者性别和肿瘤类型中，基因型和/或等位基因与甲状腺癌的发生之间无显著关联。然而，我们发现该单核苷酸多态性的等位基因和基因型分布与患者甲状腺肿瘤的大小有关。推测对病例组和对照组更多个体进行研究可能会进一步确定该单核苷酸多态性位点在伊朗阿塞拜疆人群中的基因型和等位基因频率。
