高脂肪饮食诱导的实验性大鼠模型中肺细胞外囊泡的生物发生。

High-fat diet-induced biogenesis of pulmonary exosomes in an experimental rat model.

机构信息

Pediatric Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Mol Biol Rep. 2023 Sep;50(9):7589-7595. doi: 10.1007/s11033-023-08703-y. Epub 2023 Aug 1.

DOI:10.1007/s11033-023-08703-y

PMID:37528312

Abstract

BACKGROUND

High-fat diets (HFD) have recently become a public health concern. We hypothesize that HFD induces exosomes biogenesis in the lung tissue of rat model.

METHODS AND RESULTS

Sixteen adult male Wistar rats were fed with HFD or a regular chow diet for 3 months. The histopathological changes in lung tissues were measured by hematoxylin and eosin (H&E) staining. Bronchoalveolar lavage (BAL) was performed to assay exosomes by acetylcholinesterase enzyme (AhCE) activity. Real-time PCR (qPCR) was used to evaluate Rab27-b, Alix, and IL-1β expression, while the immunohistochemical examination was performed for CD81 expression in lung tissues. In addition, expression of IL-1β was detected by ELISA. We found pathological alterations in the lung tissue of HFD animals. AhCE activity along with the expression level of Rab27-b, Alix, and IL-1β was increased in HFD animals (p < 0.05). Immunohistochemical staining showed that expression of CD81 was increased in lung tissues of HFD animals compared with the control group (p < 0.05).

CONCLUSION

Hence, HFD induced exosomes biogenesis and histopathological changes with IL-1β expression in rats' lung tissues.

摘要

背景

高脂肪饮食（HFD）最近成为公共卫生关注的焦点。我们假设 HFD 会诱导大鼠模型肺组织中的外泌体生物发生。

方法和结果

16 只成年雄性 Wistar 大鼠分别用 HFD 或常规饮食喂养 3 个月。用苏木精和伊红（H&E）染色测量肺组织的组织病理学变化。通过乙酰胆碱酯酶（AhCE）活性测定支气管肺泡灌洗液（BAL）中的外泌体。实时 PCR（qPCR）用于评估 Rab27-b、Alix 和 IL-1β 的表达，而免疫组织化学检查用于检测肺组织中 CD81 的表达。此外，通过 ELISA 检测 IL-1β 的表达。我们发现 HFD 动物的肺组织存在病理改变。HFD 动物的 AhCE 活性以及 Rab27-b、Alix 和 IL-1β 的表达水平均升高（p<0.05）。免疫组织化学染色显示 HFD 动物肺组织中 CD81 的表达高于对照组（p<0.05）。