Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, OK USA.
The Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK USA.
RNA Biol. 2023 Jan;20(1):539-547. doi: 10.1080/15476286.2023.2241755.
Angiotensin-converting enzyme 2 (ACE2) and several proteins have been identified as entry factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, whether long noncoding RNAs are involved in SARS-CoV-2 entry remains unknown. In this study, we investigated the role of small nucleolar RNA host gene 15 (SNHG15) in SARS-CoV-2 entry using a SARS-CoV-2 spike pseudotyped lentivirus with a luciferase reporter. Overexpression of SNHG15 promoted but SNHG15 knockdown limited SARS-CoV-2 entry in a dose- and time-dependent manner. SNHG15 interacted with Rab-like protein 2A (RABL2A). Overexpression and knockdown of RABL2A produced similar effects on SARS-CoV-2 entry as those of SNHG15. Furthermore, RABL2A knockdown abolished the SNHG15-mediated increase in SARS-CoV-2 entry. In conclusion, SNHG15 is a critical regulatory factor that aids SARS-CoV-2 entry through RABL2A.
血管紧张素转换酶 2（ACE2）和几种蛋白质已被鉴定为严重急性呼吸系统综合症冠状病毒 2（SARS-CoV-2）的进入因素。然而，长非编码 RNA 是否参与 SARS-CoV-2 的进入仍然未知。在这项研究中，我们使用带有荧光素酶报告基因的 SARS-CoV-2 刺突假型慢病毒，研究了小核仁 RNA 宿主基因 15（SNHG15）在 SARS-CoV-2 进入中的作用。SNHG15 的过表达促进了，但 SNHG15 的敲低以剂量和时间依赖的方式限制了 SARS-CoV-2 的进入。SNHG15 与 Rab 样蛋白 2A（RABL2A）相互作用。RABL2A 的过表达和敲低对 SARS-CoV-2 进入的作用与 SNHG15 相似。此外，RABL2A 的敲低消除了 SNHG15 介导的 SARS-CoV-2 进入增加。总之，SNHG15 是一种关键的调节因子，通过 RABL2A 辅助 SARS-CoV-2 进入。
