Teng X C, Chia K M, Zeng L P, Ma J F
Ann Acad Med Singap. 1986 Apr;15(2):141-4.
The Biotin-Avidin System (BAS) was incorporated into enzyme-linked immunosorbent assay (ELISA) technique to increase the sensitivity of the standard ELISA for the detection of HBsAg. We compared the sensitivity of Biotin-Avidin ELISA (BA-ELISA) with monoclonal antibody reverse passive hemagglutination assay (McAB RPHA), standard ELISA and solid phase radioimmunoassay (SPRIA). BA-ELISA was most sensitive. Out of 276 healthy individuals who were HBsAg negative by polyclonal antibody RPHA (PcAB-RPHA), 10(3.60%) were HBsAg positive by the BA-ELISA. Out of 123 acute hepatitis patients who were HBsAg negative by PcAb-RPHA and anti-HBc positive by ELISA, 64(52.03%) were found to be HBsAg positive by BA-ELISA. This study indicates that enhanced sensitivity of BA-ELISA in detecting HBsAg significantly increases the correctness of diagnosis of HBV infection.
将生物素-抗生物素蛋白系统（BAS）应用于酶联免疫吸附测定（ELISA）技术中，以提高标准ELISA检测乙型肝炎表面抗原（HBsAg）的灵敏度。我们比较了生物素-抗生物素蛋白ELISA（BA-ELISA）与单克隆抗体反向被动血凝试验（McAB RPHA）、标准ELISA和固相放射免疫测定（SPRIA）的灵敏度。BA-ELISA最为灵敏。在276名经多克隆抗体RPHA（PcAB-RPHA）检测HBsAg阴性的健康个体中，有10名（3.60%）经BA-ELISA检测HBsAg呈阳性。在123名经PcAb-RPHA检测HBsAg阴性且ELISA检测抗-HBc阳性的急性肝炎患者中，有64名（52.03%）经BA-ELISA检测发现HBsAg呈阳性。本研究表明，BA-ELISA在检测HBsAg时增强的灵敏度显著提高了乙肝病毒感染诊断的准确性。
