生物素-抗生物素蛋白酶联免疫吸附测定法检测乙肝表面抗原增强敏感性的临床意义
Clinical significance of enhanced sensitivity in detecting HBsAg by Biotin-Avidin ELISA.
作者信息
Teng X C, Chia K M, Zeng L P, Ma J F
出版信息
Ann Acad Med Singap. 1986 Apr;15(2):141-4.
The Biotin-Avidin System (BAS) was incorporated into enzyme-linked immunosorbent assay (ELISA) technique to increase the sensitivity of the standard ELISA for the detection of HBsAg. We compared the sensitivity of Biotin-Avidin ELISA (BA-ELISA) with monoclonal antibody reverse passive hemagglutination assay (McAB RPHA), standard ELISA and solid phase radioimmunoassay (SPRIA). BA-ELISA was most sensitive. Out of 276 healthy individuals who were HBsAg negative by polyclonal antibody RPHA (PcAB-RPHA), 10(3.60%) were HBsAg positive by the BA-ELISA. Out of 123 acute hepatitis patients who were HBsAg negative by PcAb-RPHA and anti-HBc positive by ELISA, 64(52.03%) were found to be HBsAg positive by BA-ELISA. This study indicates that enhanced sensitivity of BA-ELISA in detecting HBsAg significantly increases the correctness of diagnosis of HBV infection.
将生物素-抗生物素蛋白系统（BAS）应用于酶联免疫吸附测定（ELISA）技术中，以提高标准ELISA检测乙型肝炎表面抗原（HBsAg）的灵敏度。我们比较了生物素-抗生物素蛋白ELISA（BA-ELISA）与单克隆抗体反向被动血凝试验（McAB RPHA）、标准ELISA和固相放射免疫测定（SPRIA）的灵敏度。BA-ELISA最为灵敏。在276名经多克隆抗体RPHA（PcAB-RPHA）检测HBsAg阴性的健康个体中，有10名（3.60%）经BA-ELISA检测HBsAg呈阳性。在123名经PcAb-RPHA检测HBsAg阴性且ELISA检测抗-HBc阳性的急性肝炎患者中，有64名（52.03%）经BA-ELISA检测发现HBsAg呈阳性。本研究表明，BA-ELISA在检测HBsAg时增强的灵敏度显著提高了乙肝病毒感染诊断的准确性。
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