一种用于检测人血清中乙肝表面抗原的嵌合抗体红细胞免疫测定法。

A chimera antibody erythroimmunoassay for detecting HBsAg in human sera.

作者信息

Chen Y, Yang S C, Luo Q H

机构信息

Department of Immunology, Institute of Infectious Diseases, Beijing PRC.

出版信息

Res Virol. 1990 May-Jun;141(3):337-42. doi: 10.1016/0923-2516(90)90005-4.

DOI:10.1016/0923-2516(90)90005-4

PMID:2203124

Abstract

A highly efficient chimera antibody, a monoclonal anti-hepatitis-B-surface (anti-HBs) antibody coupled with polyclonal anti-sheep-red-blood-cell (anti-SRBC) antibody was prepared using a heterobifunctional reagent, N-succinimidyl-3-(2-pyridyldithiopropionate) (SPDP). Using SRBC as a marker, we established a sensitive solid-phase chimera antibody erythroimmunoassay (CAEIA) according to Guesdon's method. The sensitivity of this assay was 2-20 times higher than the reverse passive haemagglutination assay (RPHA) for detecting HBsAg in serially diluted sera from 10 hepatitis B patients. The weakest quantity of HBsAg detected by this assay was 4.5 ng/ml, while RPHA was unable to detect less than 75 ng/ml of HBsAg. The assay was as sensitive as the enzyme-linked immunosorbent assay (ELISA) and gave more accurate, reproducible and stable results than ELISA; specificity was also satisfactory.

摘要

使用异双功能试剂N-琥珀酰亚胺基-3-(2-吡啶基二硫代)丙酸酯（SPDP）制备了一种高效嵌合抗体，即与多克隆抗绵羊红细胞（抗SRBC）抗体偶联的单克隆抗乙型肝炎表面（抗HBs）抗体。以SRBC作为标记物，根据盖斯东方法建立了一种灵敏的固相嵌合抗体红细胞免疫测定法（CAEIA）。在对10例乙型肝炎患者的系列稀释血清进行HBsAg检测时，该测定法的灵敏度比反向被动血凝试验（RPHA）高2至20倍。该测定法检测到的HBsAg最弱量为4.5 ng/ml，而RPHA无法检测到低于75 ng/ml的HBsAg。该测定法与酶联免疫吸附测定法（ELISA）一样灵敏，并且比ELISA给出更准确、可重复和稳定的结果；特异性也令人满意。