Pôle Biologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.
UMR, Université Rennes, INSERM, Établissement Français du Sang, Rennes, France.
Front Immunol. 2023 Jul 17;14:1207545. doi: 10.3389/fimmu.2023.1207545. eCollection 2023.
Sjögren syndrome (SjS) is an autoimmune disease characterized by the destruction of the exocrine gland epithelia, causing a dryness of mucosa called sicca symptoms, and whose main life-threatening complication is lymphoma. There is a need for new biomarkers in this disease, notably diagnostic biomarkers for patients with genuine sicca symptoms that do not meet current criteria, and prognostic biomarkers for patients at risk of lymphoma. Plasma extracellular vesicles (EVs) are promising biomarker candidates in several diseases, but their potential has not yet been explored in SjS. In this proof-of-concept study, we characterized EVs from primary SjS patients (pSS, n=12) at the phenotypic and proteomic levels, compared to EVs from healthy donor (HD, n=8) and systemic lupus erythematosus patients (SLE, n=12). Specific plasma EVs subpopulations, derived from neutrophils, endothelial, and epithelial cells, were found increased in pSS. We also identified a pSS proteomic signature in plasma EVs, including neutrophil-, epithelial-, and endothelial-related proteins, such as integrin alpha M (ITGAM), olfactomedin-4 (OLFM4), Ras-related protein RAB10, and CD36. Overall, our results support the relevance of plasma EVs as biomarkers in SjS.
干燥综合征（SjS）是一种自身免疫性疾病，其特征为外分泌腺体上皮细胞的破坏，导致黏膜干燥，出现干燥症状，其主要危及生命的并发症为淋巴瘤。该疾病需要新的生物标志物，特别是用于诊断那些具有真实干燥症状但不符合当前标准的患者的生物标志物，以及用于诊断有患淋巴瘤风险的患者的预后生物标志物。血浆细胞外囊泡（EVs）是几种疾病中有前途的生物标志物候选物，但它们在 SjS 中的潜在作用尚未得到探索。在这项概念验证研究中，我们从原发性 SjS 患者（pSS，n=12）中在表型和蛋白质组学水平上对 EVs 进行了表征，并与健康供体（HD，n=8）和系统性红斑狼疮患者（SLE，n=12）的 EVs 进行了比较。发现源自中性粒细胞、内皮细胞和上皮细胞的特定血浆 EV 亚群在 pSS 中增加。我们还在血浆 EVs 中鉴定到了一个 SjS 蛋白质组特征，其中包括中性粒细胞、上皮细胞和内皮细胞相关蛋白，如整合素 alpha M（ITGAM）、嗅觉素 4（OLFM4）、Ras 相关蛋白 RAB10 和 CD36。总体而言，我们的结果支持血浆 EVs 作为 SjS 生物标志物的相关性。
