用于前列腺癌预后诊断的TMPRSS2-ERG和AR mRNA的手持式ISFET芯片实验室检测
Handheld ISFET Lab-on-Chip detection of TMPRSS2-ERG and AR mRNA for prostate cancer prognostics.
作者信息
Broomfield Joseph, Kalofonou Melpomeni, Franklin Sylvia, Powell Sue M, Pataillot-Meakin Thomas, Moser Nicolas, Bevan Charlotte L, Georgiou Pantelis
机构信息
Centre for BioInspired Technology, Electrical and Electronic Engineering, Imperial College London, UK, SW7 2AZ.
Imperial Centre for Translational and Experimental Medicine, Department of Surgery and Cancer, Imperial College London, UK, W12 0NN.
出版信息
IEEE Sens Lett. 2023 Aug;7(8):1-4. doi: 10.1109/LSENS.2017.0000000.
Abstract
Ion-sensitive field-effect transistors (ISFETs) in combination with unmodified complementary metal oxide semiconductors present a point-of-care platform for clinical diagnostics and prognostics. This work illustrates the sensitive and specific detection of two circulating mRNA markers for prostate cancer, the androgen receptor and the TMPRSS2-ERG fusion using a target-specific loop-mediated isothermal amplification method. TMPRSS2-ERG and androgen receptor RNA were detected down to 3x10 and 5x10 copies respectively in under 30 minutes. Administration of these assays onto the ISFET Lab-on-chip device was successful and the specificity of each marker was corroborated with mRNA extracted from prostate cancer cell lines.
摘要
离子敏感场效应晶体管（ISFET）与未修饰的互补金属氧化物半导体相结合，为临床诊断和预后提供了一个即时检测平台。这项工作展示了使用目标特异性环介导等温扩增法对前列腺癌的两种循环mRNA标志物——雄激素受体和TMPRSS2-ERG融合基因进行灵敏且特异的检测。在不到30分钟的时间内，分别检测到低至3×10和5×10拷贝的TMPRSS2-ERG和雄激素受体RNA。将这些检测方法应用于ISFET芯片实验室设备取得了成功，并且从前列腺癌细胞系中提取的mRNA证实了每个标志物的特异性。
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