Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua, P. R. China/School of Innovation and Entrepreneurship, Huaihua University, Huaihua, P. R. China.
Department of Pathology, Hunan University of Medicine; Huaihua, Hunan, P. R. China.
Pak J Pharm Sci. 2023 Mar;36(2):379-385.
Lycorine, a benzylphenanthridine-type alkaloid extracted form Amarillidaceae genera, exhibits an efficacy against various types of cancer. Nonetheless, the impact of lycorine treatment on neuroblastoma has not yet been investigated. Here we utilized a combinatorial strategy to explore and to understand the effect of lycorine on neuroblastoma Neuro-2a cells. Our results indicated that lycorine inhibits the Neuro-2a cells proliferation by promoting cell apoptosis. In addition, wound healing assay revealed that lycorine inhibits the Neuo-2a cells migration. Comparative transcriptome analysis showed that lycorine has the potential to affect cycle pathway. Flow cytometry analysis confirmed that lycorine arrested the Neuro-2a cell cycle at G2/M phase. Furthermore, we detected that the protein expression of Cyclin A, Cyclin B1 and Cyclin E were decreased, whereas protein of p53, Tgfβ3, Gadd45β, Gadd45γ, p21 and p27 were increased after treatment with lycorine. Collectively, we propose that lycorine might be a valuable candidate therapeutic agent in combating neuroblastoma.
石蒜碱是从石蒜科中提取的一种苯并菲啶型生物碱，具有抗多种类型癌症的功效。然而，石蒜碱治疗神经母细胞瘤的影响尚未被研究。在这里，我们采用组合策略来探索和理解石蒜碱对神经母细胞瘤 Neuro-2a 细胞的影响。我们的结果表明，石蒜碱通过促进细胞凋亡来抑制 Neuro-2a 细胞的增殖。此外，划痕愈合实验表明石蒜碱抑制 Neuo-2a 细胞的迁移。比较转录组分析表明，石蒜碱有可能影响细胞周期途径。流式细胞术分析证实石蒜碱将 Neuro-2a 细胞周期阻滞在 G2/M 期。此外，我们检测到石蒜碱处理后细胞周期蛋白 A、B1 和 E 的蛋白表达减少，而 p53、Tgfβ3、Gadd45β、Gadd45γ、p21 和 p27 的蛋白表达增加。总之，我们提出石蒜碱可能是治疗神经母细胞瘤的有价值的候选治疗剂。
