Centre for Planetary Health and Food Security (CPHFS), Nathan Campus, Griffith University, Nathan, QLD 4111, Australia.
School of Environment and Science (ESC), Nathan Campus, Griffith University, Nathan, QLD 4111, Australia.
Phytopathology. 2024 Jan;114(1):7-20. doi: 10.1094/PHYTO-05-23-0181-RVW. Epub 2024 Feb 14.
Sugarcane ( hybrid) is an important cash crop grown in tropical and subtropical countries. Ratoon stunting disease (RSD), caused by a xylem-inhabiting bacterium, subsp. () is one of the most economically significant diseases globally. RSD results in severe yield losses because its highly contagious nature and lack of visually identifiable symptoms make it harder to devise an effective management strategy. The efficacy of current management practices is hindered by implementation difficulties caused by lack of resources, high cost, and difficulties in monitoring. Rapid detection of the causal pathogen in vegetative planting material is crucial for sugarcane growers to manage this disease. Several microscopic, serological, and molecular-based methods have been developed and used for detecting the RSD pathogen. Although these methods have been used across the sugarcane industry worldwide to diagnose , some lack reliability or specificity, are expensive and time-consuming to apply, and most of all, are not suitable for on-farm diagnosis. In recent decades, there has been significant progress in the development of integrated isothermal amplification-based microdevices for accurate human and plant pathogen detection. There is a significant opportunity to develop a novel diagnostic method that integrates nanobiosensing with isothermal amplification within a microdevice format for accurate detection. In this review, we summarize (i) the historical background and current knowledge of sugarcane ratoon stunting disease, including some aspects related to transmission, pathosystem, and management practices; and (ii) the drawbacks of current diagnostic methods and the potential for application of advanced diagnostics to improve disease management.
甘蔗（杂种）是热带和亚热带国家种植的一种重要经济作物。由木质部栖息细菌引起的宿根矮化病（RSD）是全球最重要的经济意义疾病之一。RSD 会导致严重的产量损失，因为它具有高度传染性且缺乏明显的可识别症状，这使得制定有效的管理策略变得更加困难。由于资源匮乏、成本高以及监测困难，当前管理实践的效果受到阻碍。在营养繁殖材料中快速检测致病病原体对于甘蔗种植者管理这种疾病至关重要。已经开发并使用了几种基于微观、血清学和分子的方法来检测 RSD 病原体。尽管这些方法已在全球甘蔗产业中用于诊断 ，但有些方法缺乏可靠性或特异性，昂贵且耗时，最重要的是，不适合在农场进行诊断。近几十年来，基于等温扩增的集成微器件在准确的人类和植物病原体检测方面取得了重大进展。有机会开发一种新的诊断方法，该方法将纳米生物传感与微器件格式中的等温扩增相结合，用于准确 检测。在这篇综述中，我们总结了（i）甘蔗宿根矮化病的历史背景和当前知识，包括与传播、病理系统和管理实践相关的一些方面；以及（ii）当前诊断方法的缺点以及应用先进诊断方法改善疾病管理的潜力。
