Division of Biological Sciences, Wonkwang University, Iksan, Korea.
Division of Applied Life Science (BK21 four) and Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, Korea.
Plant Biotechnol J. 2023 Dec;21(12):2458-2472. doi: 10.1111/pbi.14141. Epub 2023 Aug 2.
Numerous staple crops exhibit polyploidy and are difficult to genetically modify. However, recent advances in genome sequencing and editing have enabled polyploid genome engineering. The hexaploid black nightshade species Solanum nigrum has immense potential as a beneficial food supplement. We assembled its genome at the scaffold level. After functional annotations, we identified homoeologous gene sets, with similar sequence and expression profiles, based on comparative analyses of orthologous genes with close diploid relatives Solanum americanum and S. lycopersicum. Using CRISPR-Cas9-mediated mutagenesis, we generated various mutation combinations in homoeologous genes. Multiple mutants showed quantitative phenotypic changes based on the genotype, resulting in a broad-spectrum effect on the quantitative traits of hexaploid S. nigrum. Furthermore, we successfully improved the fruit productivity of Boranong, an orphan cultivar of S. nigrum suggesting that engineering homoeologous genes could be useful for agricultural improvement of polyploid crops.
许多主要农作物表现出多倍体现象，难以进行基因修饰。然而，近年来基因组测序和编辑技术的进步使得多倍体基因组工程成为可能。六倍体黑茄物种 Solanum nigrum 作为有益的食物补充具有巨大的潜力。我们在支架水平上组装了它的基因组。在功能注释之后，我们根据与近二倍体亲缘种 Solanum americanum 和 S. lycopersicum 的同源基因进行比较分析，确定了具有相似序列和表达谱的同系基因集。使用 CRISPR-Cas9 介导的诱变，我们在同系基因中产生了各种突变组合。根据基因型，多个突变体显示出定量表型变化，导致六倍体 S. nigrum 的数量性状产生广谱效应。此外，我们成功地提高了 Boranong 的果实产量，Boranong 是 S. nigrum 的一个孤儿品种，这表明工程同系基因可能对多倍体作物的农业改良有用。
