Department of Biological Science, Yarmouk University, Irbid, Jordan.
Department of Pharmaceutics and Pharmaceutical Technology, Yarmouk University, Irbid, Jordan.
Drug Dev Ind Pharm. 2023 Aug;49(8):508-520. doi: 10.1080/03639045.2023.2243334. Epub 2023 Aug 11.
This study aims to investigate the acute and chronic adverse effects of ∼50 nm (nanometer) gold nanoparticles (AuNPs) synthesized using leaf extract in mice.
AuNPs have shown promise for medical applications, but their safety and biocompatibility need to be addressed. Understanding the potential adverse effects of AuNPs is crucial to ensure their safe use in medical applications.
The ∼50 nm AuNPs were synthesized using leaf extract and characterized using scanning electron microscopy, dynamic light scattering, and zeta potential analysis. Mice were subjected to a single intraperitoneal injection of AuNPs at a dose of 1 g/mg (grams per milligram) or a daily dose of 1 mg/kg for 28 days. Various parameters, including gold bioaccumulation, survival, behavior, body weight, and blood glucose levels, were measured. Histopathological changes and organ indices were assessed.
Gold levels in the blood and heart did not significantly increase with daily administration of AuNPs. However, there were proportional increases in gold content observed in the liver, spleen, and kidney, indicating effective tissue uptake. Histopathological alterations were predominantly observed in the kidney, suggesting potential tissue injury.
The findings of this study indicate that ∼50 nm AuNPs synthesized using leaf extract can induce adverse effects, particularly in the kidney, in mice. These results highlight the importance of addressing safety concerns when using AuNPs in medical applications. Further investigations that encompass a comprehensive set of toxicological parameters are necessary to confirm the long-term adverse effects of AuNP exposure.
本研究旨在探究使用 叶提取物合成的约 50nm（纳米）金纳米粒子（AuNPs）在小鼠体内的急性和慢性不良反应。
AuNPs 在医学应用中显示出了很大的应用前景，但它们的安全性和生物相容性需要得到解决。了解 AuNPs 的潜在不良反应对于确保其在医学应用中的安全使用至关重要。
使用 叶提取物合成约 50nm 的 AuNPs，并通过扫描电子显微镜、动态光散射和zeta 电位分析对其进行表征。小鼠经腹腔单次注射 AuNPs 剂量为 1g/mg（每毫克克）或每日剂量为 1mg/kg，持续 28 天。测量了包括金生物累积、存活率、行为、体重和血糖水平在内的各种参数。评估了组织病理学变化和器官指数。
每日给予 AuNPs 后，血液和心脏中的金水平没有显著增加。然而，在肝脏、脾脏和肾脏中观察到金含量的比例增加，表明有效组织摄取。主要在肾脏中观察到组织病理学改变，表明潜在的组织损伤。
本研究的结果表明，使用 叶提取物合成的约 50nm 的 AuNPs 可在小鼠体内引起不良反应，特别是在肾脏中。这些结果强调了在医学应用中使用 AuNPs 时需要解决安全问题的重要性。需要进一步进行涵盖一系列全面的毒理学参数的研究，以确认 AuNP 暴露的长期不良反应。
