Faculdade de Medicina Veterinária, Universidade Estadual Paulista (UNESP), Araçatuba, Clóvis Pestana St., 793, 16050-680, Araçatuba, São Paulo, Brazil.
Parasitol Res. 2019 Jul;118(7):2183-2191. doi: 10.1007/s00436-019-06342-6. Epub 2019 May 10.
Cryptosporidiosis is an emergent zoonotic disease caused by the globally distributed protozoa Cryptosporidium spp. Although several Cryptosporidium studies related to humans and many animal species have been published, there are still limited studies on the epidemiology of Cryptosporidium infection in bats. The aim of this study was to determine the occurrence of Cryptosporidium spp. and to perform the molecular characterization of Cryptosporidium species and genotypes in fecal samples from bats in an urban area of the municipality of Araçatuba, state of São Paulo, Brazil. Nested PCR targeting the 18S rRNA, actin, and HSP-70 genes was performed to screen 141 fecal samples from bats and detected Cryptosporidium spp. in 16.3% (23/141) of the samples. Bidirectional sequencing identified three novel Cryptosporidium bat genotypes (XVI, XVII, and XVIII) and a new genotype (18SH) genetically similar to Cryptosporidium avium in six species of bats. This is the first report on the occurrence and molecular characterization of Cryptosporidium spp. in Brazilian bats. Zoonotic Cryptosporidium species were not found in fecal samples from bats living in an urban area in the municipality of Araçatuba, state of São Paulo, Brazil.
隐孢子虫病是一种由分布广泛的原生动物隐孢子虫属引起的新兴人畜共患病。尽管已经发表了许多与人类和许多动物物种有关的隐孢子虫研究，但关于蝙蝠中隐孢子虫感染的流行病学研究仍然有限。本研究旨在确定隐孢子虫属的发生情况，并对来自巴西圣保罗州阿拉萨图巴市城区蝙蝠粪便样本中的隐孢子虫种和基因型进行分子特征分析。针对 18S rRNA、肌动蛋白和 HSP-70 基因的巢式 PCR 用于筛查 141 份蝙蝠粪便样本，结果在 16.3%（23/141）的样本中检测到隐孢子虫属。双向测序鉴定出三种新型蝙蝠隐孢子虫基因型（XVI、XVII 和 XVIII）和一种新型基因型（18SH），与巴西圣保罗州阿拉萨图巴市城区蝙蝠粪便样本中的六种种蝙蝠中的空肠隐孢子虫具有遗传相似性。这是首次报道巴西蝙蝠中隐孢子虫属的发生和分子特征分析。在巴西圣保罗州阿拉萨图巴市城区蝙蝠的粪便样本中未发现粪源性隐孢子虫属。
