- Universidade Federal do Rio de Janeiro, Departamento de Cirurgia Experimental - Rio de Janeiro - RJ - Brasil.
- Hospital Naval Marcílio Dias - Rio de Janeiro - RJ - Brasil.
Rev Col Bras Cir. 2023 Jul 28;50:e20233453. doi: 10.1590/0100-6991e-20233453-en. eCollection 2023.
this research objective was to develop a new peritoneal adhesion animal model that would lead to adhesions formation in all operated animals, simple and reproducible, associated with maintenance the animal's health.
eighteen adult male Wistar rats (Rattus norvegicus) were randomly distributed into three groups: Control Group (anatomical and clinical parameters), Sham Group (delicate manipulation of the stomach and exposure of the peritoneal cavity to ambient air) and Surgery Group (gastrotomy followed by gastrorrhaphy). The animals were analyzed and classificated macroscopically according to two adhesion classification models and differences between groups were considered significant when p<0.05.
the six animals in the control group had no peritoneal adhesions, three of the six animals in the sham group had focal peritoneal adhesions, and all animals in the surgery group (gastrotomy followed by gastrorraphy) had firm peritoneal adhesions. All adhesions found were macroscopically quantified and microscopically confirmed, without carrying out a microscopic classification of the adhesions.
the new model developed of gastrotomy followed by gastrorrhaphy, proved to be safe and efficient to induce and study peritoneal adhesions.
本研究旨在建立一种新的腹膜粘连动物模型，该模型能够在所有手术动物中引发粘连形成，操作简单，可重现，同时保持动物健康。
18 只成年雄性 Wistar 大鼠（Rattus norvegicus）随机分为三组：对照组（解剖和临床参数）、假手术组（胃的精细操作和暴露于腹腔空气）和手术组（胃切开术加胃缝合术）。根据两种粘连分类模型对动物进行宏观分析和分类，当 p<0.05 时，认为组间差异具有统计学意义。
对照组的 6 只动物无腹膜粘连，假手术组的 6 只动物中有 3 只出现局限性腹膜粘连，手术组（胃切开术加胃缝合术）的所有动物均出现牢固的腹膜粘连。所有发现的粘连均进行了宏观量化，并通过显微镜确认，未对粘连进行微观分类。
新建立的胃切开术加胃缝合术模型，被证明是一种安全有效的诱导和研究腹膜粘连的方法。
