洗必泰和茶树油对减少口腔微生物数量的影响。

Effect of Chlorhexidine and Tea Tree Oil on Reducing the Number of Oral Microorganisms.

作者信息

Moreno André Luiz de Melo, de Moraes Melo Neto Clóvis Lamartine, Coelho Goiato Marcelo, Moreno Nathaly Vilene de Araujo, Dos Santos Daniela Micheline, de Lima Cássia Cunha, Lopes Motta Rogério Heládio, Ramacciato Juliana Cama

机构信息

Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil.

Oral Oncology Center, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil.

出版信息

Eur J Dent. 2024 Feb;18(1):397-400. doi: 10.1055/s-0043-1769900. Epub 2023 Aug 2.

DOI:10.1055/s-0043-1769900

PMID:37532117

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10959613/

Abstract

OBJECTIVES

Thus, the aim of this study was to compare the effect of using two preoperative mouthwashes (0.12% chlorhexidine and 0.2% tea tree oil) on the number of colonies of oral microorganisms.

MATERIALS AND METHODS

Forty participants who needed to be rehabilitated with dental implants were included in this study. They were randomly divided into two groups (chlorhexidine group and tea tree group; = 20, each). For each group, saliva samples were collected at four different times: T0 (initially)-before using the mouthwash, T1-after 1 minute of using the mouthwash, T10-after 10 minutes of using the mouthwash, and T60-after 60 minutes of using the mouthwash. At T0 and T1, saliva samples were collected before implant placement surgery, and at T10 and T60, saliva samples were collected during surgery. In each group, one saliva sample was collected at each evaluated time point for each patient, totaling 4 saliva collections per patient. MSB agar (Mitis-Salivarius-Bacitracin) and BHI agar (Brain Heart Infusion) culture media were used in each group. Microbial colony counts were performed using a magnifying glass and recorded in CFU (colony forming units)/mL. Statistical analyses were performed using the Friedman, Mann-Whitney U and Wilcoxon tests ( < 0.05).

RESULTS

Based on MSB agar culture medium, at T0, the number of colonies in the chlorhexidine group was significantly higher compared with the tea tree group ( <0.05; MSB agar). The chlorhexidine group showed significantly lower CFU/mL values for at T1, T10, and T60 compared with the tea tree group ( <0.05; MSB agar). Based on BHI agar culture medium, at T0, the chlorhexidine group showed a significantly lower value of CFU/mL compared with the tea tree group ( < 0.05; BHI agar). At T1, T10, and T60, the chlorhexidine group showed significantly lower CFU/mL values compared with the tea tree group ( <0.05; BHI agar).

CONCLUSION

Chlorhexidine is more indicated as a preoperative mouthwash than tea tree oil, due to its significantly more effective antimicrobial action.

摘要

目的

因此，本研究的目的是比较两种术前漱口水（0.12%洗必泰与0.2%茶树油）对口腔微生物菌落数量的影响。

材料与方法

本研究纳入了40名需要进行牙种植修复的参与者。他们被随机分为两组（洗必泰组和茶树组；每组n = 20）。对于每组，在四个不同时间点采集唾液样本：T0（初始）——使用漱口水前，T1——使用漱口水1分钟后，T10——使用漱口水10分钟后，以及T60——使用漱口水60分钟后。在T0和T1时，在种植体植入手术前采集唾液样本，在T10和T60时，在手术过程中采集唾液样本。在每组中，为每位患者在每个评估时间点采集一份唾液样本，每位患者共采集4份唾液样本。每组均使用MSB琼脂（轻唾-杆菌肽琼脂）和BHI琼脂（脑心浸液琼脂）培养基。使用放大镜进行微生物菌落计数，并记录为CFU（菌落形成单位）/mL。采用Friedman检验、Mann-Whitney U检验和Wilcoxon检验进行统计分析（P < 0.05）。

结果

基于MSB琼脂培养基，在T0时，洗必泰组的菌落数量显著高于茶树组（P < 0.05；MSB琼脂）。与茶树组相比，洗必泰组在T1、T10和T60时的CFU/mL值显著更低（P < 0.05；MSB琼脂）。基于BHI琼脂培养基，在T0时，洗必泰组的CFU/mL值显著低于茶树组（P < 0.05；BHI琼脂）。在T1、T10和T60时，洗必泰组的CFU/mL值显著低于茶树组（P < 0.05；BHI琼脂）。