Bansal Aayushi, Marwah Nikhil, Nigam Anant Gopal, Goenka Puneet, Goel Deepak
Senior Resident, Department of Pedodontics and Preventive Dentistry, RUHS College of Dental Sciences, Jaipur, Rajasthan, India, e-mail:
Department of Pedodontics and Preventive Dentistry, Mahatma Gandhi Dental College and Hospital, Jaipur, Rajasthan, India.
J Contemp Dent Pract. 2015 Nov 1;16(11):903-9. doi: 10.5005/jp-journals-10024-1779.
To study the effect Achyranthes aspera, 0.2% aqueous chlorhexidine gluconate and Punica granatum oral rinse on salivary Streptococcus mutans count in children.
A total of 60 children of 8 to 12 years of age were randomly allocated into 3 groups. Group A was given 0.2% chlorhexidine mouthwash, group B was given 10% A. aspera mouthwash and group C was given 15% P. granatum mouthwash. The day 1 saliva samples were collected from the subjects and inoculated onto mitis salivarius bacitracin (MSB) agar. The colony counts were obtained by a clinical microbiologist who was blinded to the subject allocation. Plaque scores were then recorded by the investigator with the help of a volunteer. Following this, they received a thorough scaling and polishing. Subjects in each group were then provided with 140 ml of the respective mouthwash, as a daily supervised rinse after breakfast and before sleeping as per instructions. Following mouth rinsing, the children were instructed not to eat or drink for 15 minutes. At the 7th day, unstimulated saliva was again collected from the subjects of all 3 groups, inoculated onto MSB agar and colony count was obtained. Modified Quigley-Hein plaque index was also evaluated for the refreshed score at this stage. Colony counting was done using loop method and statistical analysis was done using Statistical Package for the Social Science (SPSS) software version 21.
All the three mouthwashes showed statistically significant reduction of S. mutans count and plaque index after 7 days, i.e. chlorhexidine (p < 0.001 for reduction in S. mutans count and p < 0.05 for plaque score reduction), A. aspera (p < 0.01 for reduction in S. mutans count and p < 0.05 for plaque score reduction) and P. granatum (p < 0.01 for reduction in S. mutans count and p < 0.05 for plaque score reduction). Chlorhexidine had marginally better results in reducing S. mutans count.
Efficacy of chlorhexidine, A. aspera and P. granatum was statistically significant with respect to reduction of S. mutans count with chlorhexidine being marginally better than the other two; All the three mouthwashes were found to be at par when plaque index values from baseline and after interception of 7 days was calculated; Punica granatum has better antimicrobial effect than A. aspera.
研究牛膝、0.2%葡萄糖酸氯己定水溶液和石榴皮含漱液对儿童唾液中变形链球菌数量的影响。
将60名8至12岁的儿童随机分为3组。A组给予0.2%氯己定漱口水，B组给予10%牛膝漱口水，C组给予15%石榴皮漱口水。在第1天采集受试者的唾液样本并接种到唾液链球菌杆菌肽（MSB）琼脂上。由对受试者分组不知情的临床微生物学家进行菌落计数。然后研究者在一名志愿者的帮助下记录菌斑评分。在此之后，他们接受了彻底的洁治和抛光。然后每组受试者按照指示，每天在早餐后和睡前接受监督使用140毫升各自的漱口水进行含漱。含漱后，指示儿童15分钟内不吃不喝。在第7天，再次从所有3组受试者中采集非刺激性唾液，接种到MSB琼脂上并获得菌落计数。在此阶段还评估了改良的Quigley - Hein菌斑指数以获得更新后的评分。使用接种环法进行菌落计数，并使用社会科学统计软件包（SPSS）21版进行统计分析。
7天后，所有三种漱口水均显示出变形链球菌数量和菌斑指数在统计学上有显著降低，即氯己定（变形链球菌数量减少p < 0.001，菌斑评分降低p < 0.05）、牛膝（变形链球菌数量减少p < 0.01，菌斑评分降低p < 0.05）和石榴皮（变形链球菌数量减少p < 0.01，菌斑评分降低p < 0.05）。氯己定在减少变形链球菌数量方面的效果略好。
氯己定、牛膝和石榴皮在减少变形链球菌数量方面的疗效具有统计学意义，氯己定略优于其他两种；当计算基线和7天干预后的菌斑指数值时，发现所有三种漱口水相当；石榴皮的抗菌效果优于牛膝。
