School of Biotechnology, Jiangsu University of Science and Technology, 212100 Zhenjiang, China.
School of Biotechnology, Jiangsu University of Science and Technology, 212100 Zhenjiang, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 212100 Zhenjiang, China.
Pestic Biochem Physiol. 2023 Aug;194:105503. doi: 10.1016/j.pestbp.2023.105503. Epub 2023 Jun 17.
Glyphodes pyloalis Walker (G. pyloalis) is a common destructive mulberry pest. Due to the long-term and frequent use of insecticides, it has developed tolerance to commonly used insecticides. Tolfenpyrad (TFP) is a novel pyrazole heterocyclic insecticide. In order to understand the TFP detoxification mechanism of G. pyloalis larvae, we first estimated the LC dose of TFP for 3 instar G. pyloalis larvae. Next, we identified genes that were differentially expressed in 3 instar G. pyloalis larvae treated with TFP compared to the control group by transcriptome sequencing. In total, 86,949,569 and 67,442,028 clean reads were obtained from TFP-treated and control G. pyloalis larvae, respectively. A total of 5588 differentially expressed genes (DEGs) were identified in TFP-treated and control G. pyloalis larvae, of which 3084 genes were upregulated and 2504 genes were downregulated. We analyzed the expression of 43 candidate detoxification enzyme genes associated with insecticide tolerance using qPCR. According to the spatiotemporal expression pattern of DEGs, we found that CYP6ABE1, CYP333A36 and GST-epsilon8 were highly expressed in the midgut, while CarEs14 was strongly expressed in haemolymph. Furthermore, we successfully knocked down these genes by RNA interference. After silencing CYP6ABE1 and CYP333A36, bioassay showed that the mortality rate of TFP-treated G. pyloalis larvae was significantly higher compared to the control group. This study provides a theoretical foundation for understanding the sensitivity of G. pyloalis to TFP and establish the basis for the effective and green management of this pest.
豆绿尺蛾（Glyphodes pyloalis）是一种常见的桑树破坏性害虫。由于长期和频繁地使用杀虫剂，它对常用杀虫剂产生了耐受性。多杀菌素（TFP）是一种新型吡唑杂环类杀虫剂。为了了解豆绿尺蛾幼虫对 TFP 的解毒机制，我们首先估算了 TFP 对 3 龄豆绿尺蛾幼虫的致死剂量。接下来，我们通过转录组测序鉴定了 TFP 处理的 3 龄豆绿尺蛾幼虫与对照组相比差异表达的基因。分别从 TFP 处理和对照的豆绿尺蛾幼虫中获得了 86949569 和 67442028 条清洁读段。在 TFP 处理和对照的豆绿尺蛾幼虫中分别鉴定到 5588 个差异表达基因（DEGs），其中 3084 个基因上调，2504 个基因下调。我们使用 qPCR 分析了与杀虫剂耐受性相关的 43 个候选解毒酶基因的表达。根据 DEGs 的时空表达模式，我们发现 CYP6ABE1、CYP333A36 和 GST-epsilon8 在中肠中高表达，而 CarEs14 在血液中强烈表达。此外，我们通过 RNA 干扰成功敲低了这些基因。沉默 CYP6ABE1 和 CYP333A36 后，生物测定表明，TFP 处理的豆绿尺蛾幼虫死亡率明显高于对照组。本研究为了解豆绿尺蛾对 TFP 的敏感性提供了理论基础，并为该害虫的有效和绿色管理奠定了基础。
