Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China; Suzhou Academy of Agricultural Sciences, Suzhou 234000, China.
Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China.
Pestic Biochem Physiol. 2023 Aug;194:105513. doi: 10.1016/j.pestbp.2023.105513. Epub 2023 Jul 1.
Riptortus pedestris (bean bug), a common soybean pest, has a highly developed olfactory system to find hosts for feeding and oviposition. Chemosensory proteins (CSPs) have been identified in many insect species; however, their functions in R. pedestris remain unknown. In this study, quantitative real time-polymerase chain reaction (qRT-PCR) revealed that the expression of RpedCSP12 in the adult antennae of R. pedestris increased with age. Moreover, a significant difference in the expression levels of RpedCSP12 was observed between male and female antennae at one and three days of age. We also investigated the binding ability of RpedCSP12 to different ligands using a prokaryotic expression system and fluorescence competitive binding assays. We found that RpedCSP12 only bound to one aggregation pheromone, (E)-2-hexenyl (Z)-3-hexenoate, and its binding decreased with increasing pH. Furthermore, homology modelling, molecular docking, and site-directed mutagenesis revealed that the Y27A, L74A, and L85A mutants lost their binding ability to (E)-2-hexenyl (Z)-3-hexenoate. Our findings highlight the olfactory roles of RpedCSP12, providing insights into the mechanism by which RpedCSPs bind to aggregation pheromones. Therefore, our study can be used as a theoretical basis for the population control of R. pedestris in the future.
豆野螟（豆荚野螟），一种常见的大豆害虫，拥有高度发达的嗅觉系统来寻找取食和产卵的宿主。化感蛋白（CSPs）已在许多昆虫物种中被鉴定出来；然而，它们在 R. pedestris 中的功能尚不清楚。在这项研究中，定量实时聚合酶链反应（qRT-PCR）显示 R. pedestris 成虫触角中 RpedCSP12 的表达随年龄增长而增加。此外，在 1 天和 3 天时，雄虫和雌虫触角中 RpedCSP12 的表达水平存在显著差异。我们还使用原核表达系统和荧光竞争结合测定研究了 RpedCSP12 与不同配体的结合能力。我们发现 RpedCSP12 仅与一种聚集信息素（E）-2-己烯基（Z）-3-己烯酸酯结合，其结合能力随 pH 值的增加而降低。此外，同源建模、分子对接和定点突变显示 Y27A、L74A 和 L85A 突变体失去了与（E）-2-己烯基（Z）-3-己烯酸酯结合的能力。我们的研究结果强调了 RpedCSP12 的嗅觉作用，为 RpedCSPs 与聚集信息素结合的机制提供了深入了解。因此，我们的研究可以为未来控制 R. pedestris 的种群提供理论依据。
