Liu Panjing, Guo Jianglong, Wei Hongyi, Feng Likai, Gao Zhanlin, Zhang Tao
Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Integrated Pest Management Center of Hebei Province, Baoding, China.
Institute of Entomology, Jiangxi Agricultural University, Nanchang, China.
Front Physiol. 2023 Jul 14;14:1224009. doi: 10.3389/fphys.2023.1224009. eCollection 2023.
A sophisticated and sensitive olfactory system plays a vital role in the survival and reproduction of insects. Chemosensory receptors are indispensable for the molecular recognition and discrimination of semiochemicals. is a notorious pest of legume plants, resulting in yield losses and quality decreases in soybeans. It is well accepted that highly relies on its olfactory system in detecting aggregation pheromones, host volatiles, and pesticides; however, little research focused on its chemosensory receptors. In the present study, we identified 237 odorant receptors (ORs), 42 gustatory receptors (GRs), and 31 ionotropic receptors (IRs) from the reported genome of , and analyzed their phylogenetic relationship with other hemipteran species. Through the results of RNA-seq and real-time quantitative PCR (qRT-PCR), we found that RpedORs displayed different expression levels in the antennae of at different development stages. To further verify the function of odorant receptor co-receptor (Orco), an obligate and unique insect OR, we silenced by RNA interference (RNAi) method. The results showed that silencing could significantly impair the response to aggregation pheromone in , indicating that plays an essential role in odorant detection. Our results can provide the theoretical foundations for revealing the olfactory recognition mechanism of and help explore and develop novel olfactory-based agents against this pest.
一个复杂而灵敏的嗅觉系统在昆虫的生存和繁殖中起着至关重要的作用。化学感受受体对于信息素的分子识别和区分是不可或缺的。[此处原文缺失某种昆虫名称]是豆类植物臭名昭著的害虫，会导致大豆产量损失和品质下降。人们普遍认为，[该昆虫名称]在检测聚集信息素、寄主挥发物和农药时高度依赖其嗅觉系统；然而，很少有研究关注其化学感受受体。在本研究中，我们从已报道的[昆虫名称]基因组中鉴定出237个气味受体（ORs）、42个味觉受体（GRs）和31个离子型受体（IRs），并分析了它们与其他半翅目物种的系统发育关系。通过RNA测序（RNA-seq）和实时定量PCR（qRT-PCR）结果，我们发现RpedORs在[昆虫名称]不同发育阶段的触角中表现出不同的表达水平。为了进一步验证气味受体共受体（Orco）（一种专一且独特的昆虫OR）的功能，我们通过RNA干扰（RNAi）方法使[相关基因名称]沉默。结果表明，沉默[相关基因名称]会显著损害[昆虫名称]对聚集信息素的反应，这表明[相关基因名称]在气味检测中起重要作用。我们的结果可为揭示[昆虫名称]的嗅觉识别机制提供理论基础，并有助于探索和开发针对这种害虫的新型嗅觉类药剂。
