Anhui-CABI Joint Laboratory for Agricultural Pest Control, Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China.
Pestic Biochem Physiol. 2024 Sep;204:106073. doi: 10.1016/j.pestbp.2024.106073. Epub 2024 Aug 5.
Riptortus pedestris (Hemiptera: Alydidae) is a notable soybean pest, with diapause and non-diapause individuals showing different sensitivities to aggregation pheromones. This study aimed to investigate how R. pedestris detects aggregation pheromones through electroantennogram (EAG) and behavioral experiments, transcriptome sequencing and qRT-PCR, as well as competitive fluorescence-binding assay. Results indicated that diapausing females and males of R. pedestris exhibited a heightened EAG response and were more attracted to the aggregation pheromone components compared to their non-diapause counterparts. Transcriptome sequencing and qRT-PCR analyses revealed significantly higher expression of RpedOBP1 in the antennae of diapause females and males compared to non-diapausing R. pedestris. The competitive fluorescence-binding assay demonstrated that RpedOBP1 displayed the strongest binding affinity to E2HE2H, suggesting its crucial role in recognizing the aggregation pheromone. These findings have the potential to inform the development of integrated pest management strategies utilizing behavioral approaches for bean bug control.
稻绿蝽（半翅目：蝽科）是一种重要的大豆害虫，滞育和非滞育个体对聚集信息素有不同的敏感性。本研究旨在通过触角电位（EAG）和行为实验、转录组测序和 qRT-PCR 以及竞争荧光结合测定，研究稻绿蝽如何检测聚集信息素。结果表明，稻绿蝽滞育雌、雄虫对聚集信息素成分的 EAG 反应增强，比非滞育个体更具吸引力。转录组测序和 qRT-PCR 分析显示，滞育雌、雄虫触角中 RpedOBP1 的表达明显高于非滞育稻绿蝽。竞争荧光结合测定表明，RpedOBP1 与 E2HE2H 表现出最强的结合亲和力，表明其在识别聚集信息素中起关键作用。这些发现有可能为利用行为方法控制豆蝽的综合害虫管理策略的制定提供信息。
