School of Forestry, Northeast Forestry University, Harbin 15004, China.
School of Forestry, Northeast Forestry University, Harbin 15004, China.
Pestic Biochem Physiol. 2023 Aug;194:105527. doi: 10.1016/j.pestbp.2023.105527. Epub 2023 Jul 12.
Bursaphelenchus xylophilus (Pine wood nematode, PWN) has become a worldwide forest disease due to its rapid infection ability, high lethality and difficulty in control. The main means of countering B. xylophilus is currently chemical control, but nematicides can present problems such as environmental pollution and drug resistance. The development of novel environmentally-friendly nematicides has thus become a focus of recent research. In this study, BxUGT3 and BxUGT34, which might be related to detoxification, were investigated by comparing transcriptomic and WGCNA approaches. Three other genes with a similar expression pattern, BxUGT13, BxUGT14, and BxUGT16, were found by gene family analysis. Further bioassays and qPCR assays confirmed that these five genes showed significant changes in transcript levels upon exposure to α-pinene and carvone, demonstrating that they respond to exogenous nematicidal substances. Finally, RNAi and bioassays showed that B. xylophilus with silenced BxUGT16 had increased mortality in the face of α-pinene and carvone stress, suggesting that BxUGT16 plays an important role in detoxification. Taken together, this study used novel molecular research methods, explored the detoxification mechanism of B. xylophilus at a transcriptomic level, and revealed a molecular target for the development of novel biopesticides.
松材线虫（Pine wood nematode, PWN）因其快速的感染能力、高致死率和难以控制等特点，已成为一种全球性的森林病害。目前防治松材线虫的主要手段是化学防治，但杀线虫剂可能会带来环境污染和抗药性等问题。因此，开发新型环保型杀线虫剂已成为当前研究的焦点。在本研究中，我们通过比较转录组学和 WGCNA 方法，研究了可能与解毒相关的 BxUGT3 和 BxUGT34。通过基因家族分析，我们还发现了另外三个具有相似表达模式的基因 BxUGT13、BxUGT14 和 BxUGT16。进一步的生物测定和 qPCR 分析证实，这五个基因在暴露于α-蒎烯和香芹酮时转录水平发生显著变化，表明它们对外源杀线虫物质有反应。最后，RNAi 和生物测定表明，沉默 BxUGT16 的松材线虫在面对α-蒎烯和香芹酮胁迫时死亡率增加，表明 BxUGT16 在解毒中发挥重要作用。综上所述，本研究采用了新颖的分子研究方法，在转录组水平上探讨了松材线虫的解毒机制，并揭示了一种新型生物农药开发的分子靶标。
