Supplementary General Sciences Department, Faculty of Oral and Dental Medicine, Future University in Egypt, Cairo, 11835, Egypt.
Department of Clinical Pharmacy, College of Pharmacy, Jazan University, 45142, Jazan, Saudi Arabia.
Sci Rep. 2023 Aug 2;13(1):12522. doi: 10.1038/s41598-023-39385-y.
Solid lipid nanoparticles second generation, nanostructure lipid carrier (NLC), is one of the most important biodegradable nanoparticles. Nanostructure Lipid carrier (NLC) was used to encapsulate methylene blue (MB) dye, carvacrol and citronellal and their efficacy as insecticidal against Culex pipiens (Cx. pipiens) were distinguished. The prepared nanoformulation revealed very good physicochemical properties, especially the homogeneity of the particle size. Transmission electron microscope showed spherical shaped nanoparticles within range less than 200 nm. The prepared NLC-MB-MT system showed a very competitive insecticidal activity and high virulence against the mosquito larvae with higher mortality rate of LC of 0.141 µl/mL, in addition to high level of Oxidative stress parameters obtained through all the tested enzymes including hydrogen peroxide (4.8 ppm), protein carbonyl amount (0.12 OD/mg protein), ascorbic acid (0.15 mg) and Superoxide dismutase (SOD) showed strong increasing (0.09 OD/mg protein/min) at 6 µg/mL, respectively. Whereas paradoxical results of the oxidative stress enzymes were obtained from different concentration of nanoformulation that introduce a convenient reason for their potential insecticidal effect. The cytotoxic effect of NLC-MB-MT was evaluated using WI38 human lung cell lines, the LC was 6.4 mg/mL. The low cytotoxic reactivity towards the tested cell line makes the NLC-MB-MT nanoformulation has its promising insecticidal efficacy. Molecular docking study for each component were done against acetylcholine esterase protein and accepted binding modes achieved by the three compounds.
第二代固体脂质纳米粒，即纳米结构脂质载体（NLC），是最重要的可生物降解纳米颗粒之一。纳米结构脂质载体（NLC）被用于包封亚甲蓝（MB）染料、香芹酚和香茅醛，并比较了它们作为杀虫剂对库蚊（Cx. pipiens）的功效。所制备的纳米制剂表现出非常好的物理化学性质，特别是粒径的均一性。透射电子显微镜显示出球形的纳米颗粒，粒径范围小于 200nm。所制备的 NLC-MB-MT 系统表现出非常有竞争力的杀虫活性和对蚊幼虫的高毒性，LC 为 0.141µl/mL，死亡率更高，此外，通过所有测试的酶获得的氧化应激参数水平很高，包括过氧化氢（4.8ppm）、蛋白质羰基含量（0.12 OD/mg 蛋白）、抗坏血酸（0.15mg）和超氧化物歧化酶（SOD），在 6µg/mL 时分别表现出强烈的增加（0.09 OD/mg 蛋白/分钟）。然而，不同浓度的纳米制剂中获得了氧化应激酶的矛盾结果，这为它们的潜在杀虫效果提供了一个方便的原因。用 WI38 人肺细胞系评估了 NLC-MB-MT 的细胞毒性作用，LC 为 6.4mg/mL。该纳米制剂对测试细胞系的低细胞毒性反应使其具有有前途的杀虫功效。对每种化合物进行了针对乙酰胆碱酯酶蛋白的分子对接研究，并接受了三种化合物达到的结合模式。
