Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, China.
Arch Insect Biochem Physiol. 2023 Oct;114(2):1-16. doi: 10.1002/arch.22044. Epub 2023 Aug 2.
The Asian corn borer (Ostrinia furnacalis) is an important agricultural pest causing serious damage to economic crops, such as corn and sorghum. The gut is the first line of defense against pathogens that enter through the mouth. Staphylococcus aureus was used to infect the O. furnacalis midgut to understand the midgut immune mechanism against exogenous pathogens to provide new ideas and methods for the prevention and control of O. furnacalis. A sequencing platform was used for genome assembly and gene expression. The unigene sequences were annotated and functionally classified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Significant differences were found in the induced expression profiles before and after infection. Some differentially expressed genes have important relations with lipid metabolism and immune mechanism, suggesting that they play an important role in the innate immune response of O. furnacalis. Furthermore, quantitative real-time polymerase chain reaction assay was used to identify the key genes involved in the signaling pathway, and the expression patterns of these key genes were confirmed. The results could help study the innate immune system of lepidopteran insects and provide theoretical support for the control of related pests and the protection of beneficial insects.
亚洲玉米螟（Ostrinia furnacalis）是一种重要的农业害虫，对玉米、高粱等经济作物造成严重损害。肠道是抵御经口进入的病原体的第一道防线。本研究采用金黄色葡萄球菌感染亚洲玉米螟中肠，解析中肠抵御外源性病原体的免疫机制，以期为亚洲玉米螟的防控提供新思路和新方法。利用测序平台进行基因组组装和基因表达分析。通过基因本体论和京都基因与基因组百科全书对 unigene 序列进行注释和功能分类。感染前后诱导表达谱存在显著差异。一些差异表达基因与脂质代谢和免疫机制密切相关，表明它们在亚洲玉米螟先天免疫反应中发挥重要作用。此外，采用实时荧光定量聚合酶链式反应检测参与信号通路的关键基因，并对这些关键基因的表达模式进行验证。该研究结果有助于研究鳞翅目昆虫的先天免疫系统，为相关害虫的防治和有益昆虫的保护提供理论支持。
