Ren Wen, Messerschmidt Victoria, Tsipursky Michael, Irudayaraj Joseph
Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
Biomedical Research Center in Mills Breast Cancer Institute, Carle Foundation Hospital, Champaign, Illinois 61801, United States.
ACS Appl Nano Mater. 2023 Jul 17;6(14):13116-13126. doi: 10.1021/acsanm.3c01812. eCollection 2023 Jul 28.
Herein, we propose an oxygen nanobubbles-embedded hydrogel (ONB-G) with carbopol for oxygenation of wounds to accelerate the wound healing process. We integrate carbopol, hydrogel, and dextran-based oxygen nanobubbles (ONBs) to prepare ONB-G where ONBs can hold and release oxygen to accelerate wound healing. Oxygen release tests showed that the proposed ONB-G could encapsulate oxygen in the hydrogels for up to 34 days; meanwhile, fluorescence studies indicated that the ONB-G could maintain high oxygen levels for up to 4 weeks. The effect of carbopol concentration on the oxygen release capacity and rheological features of the ONB-G were also investigated along with the sterility of ONB-G. HDFa cell-based studies were first conducted to evaluate the viability, proliferation, and revival of cells in hypoxia. Scratch assay and mRNA expression studies indicated the potential benefit for wound closure. Histological evaluation of tissues with a pig model with incision and punch wounds showed that treatment with ONB-G exhibited improved healing compared with hydrogel without ONBs or treated without the gel. Our studies show that dextran-shell ONBs embedded in a gel (ONB-G) have the potential to accelerate wound healing, given its oxygen-holding capacity and release properties.
在此，我们提出一种含有卡波姆的氧纳米气泡包埋水凝胶（ONB-G），用于伤口氧合以加速伤口愈合过程。我们将卡波姆、水凝胶和基于葡聚糖的氧纳米气泡（ONBs）整合在一起制备ONB-G，其中ONBs可以储存并释放氧气以加速伤口愈合。氧气释放测试表明，所提出的ONB-G可以在水凝胶中封装氧气长达34天；同时，荧光研究表明，ONB-G可以在长达4周的时间内维持高氧水平。还研究了卡波姆浓度对ONB-G的氧气释放能力和流变特性的影响以及ONB-G的无菌性。首先进行了基于人皮肤成纤维细胞（HDFa）的研究，以评估缺氧条件下细胞的活力、增殖和复苏情况。划痕试验和mRNA表达研究表明其对伤口闭合具有潜在益处。对猪的切口和打孔伤口模型组织进行的组织学评估表明，与不含ONBs的水凝胶或未用凝胶处理相比，用ONB-G处理显示出更好的愈合效果。我们的研究表明，鉴于其储氧能力和释放特性，包埋在凝胶中的葡聚糖壳ONBs（ONB-G）有加速伤口愈合的潜力。
