Tian Yongfeng, Zhao Lu, Pan Yonghua, Li Zhengfeng, Shen Xiaofeng, Zhang Xia, Tang Xianghu, Feng Xin, Huang Xingjiu
Technology Center of China Tobacco Yunnan Industrial Co., Ltd. Kunming 650231 China
Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, HFIPS, Chinese Academy of Sciences Hefei 230031 China
RSC Adv. 2023 Aug 1;13(33):23130-23137. doi: 10.1039/d3ra03977c. eCollection 2023 Jul 26.
Surface enhanced Raman spectroscopy (SERS) is a highly sensitive analytical detection technique that provides unique chemical and structural information on target molecules. Snus is a type of tobacco product that can release nicotine and other components under certain humidity and temperature without burning, and the evaluation of its nicotine release under different storage conditions is very important for understanding its characteristics, regulating its components, and setting reasonable storage conditions. Herein, by means of an artificial climate box and suction extraction device, the volatile release evaluations of nicotine from snus products under different storage conditions were performed based on FeO microparticles coated with Au nanorods and Au nanoparticles (FeO@AuNRsNPs) as SERS substrates combined with a capillary. The FeO@AuNRsNPs assemblies can be fixed in the inner wall of the capillary with the aid of an external magnetic field, which improved the maneuverability of the SERS substrates. By comparing the intensities of the spectral peaks of the symmetrical breathing of the pyridine moiety of nicotine molecules with increasing temperature and humidity, which could significantly accelerate the volatile release of a small amount of nicotine, the nicotine release under different conditions could be evaluated. Based on this strategy, it was possible to obtain the storage or placement conditions of the product. The results of this study provide a reference to clarify the volatile release of nicotine under various storage conditions, which is helpful for better regulation of the levels of nicotine in snus. Moreover, such destruction-free evaluation of the volatile release of nicotine from snus products under different storage conditions opens up new perspectives for further research about the impact of nicotinoids on smokers' health and cessation programs.
表面增强拉曼光谱（SERS）是一种高灵敏度的分析检测技术，可提供有关目标分子的独特化学和结构信息。口含烟是一种烟草制品，在一定湿度和温度下无需燃烧即可释放尼古丁和其他成分，评估其在不同储存条件下的尼古丁释放对于了解其特性、调节其成分以及设定合理的储存条件非常重要。在此，借助人工气候箱和抽吸提取装置，基于涂覆有金纳米棒和金纳米粒子的FeO微粒（FeO@AuNRsNPs）作为SERS基底并结合毛细管，对口含烟产品在不同储存条件下尼古丁的挥发性释放进行了评估。FeO@AuNRsNPs组件可借助外部磁场固定在毛细管内壁，这提高了SERS基底的可操作性。通过比较随着温度和湿度升高尼古丁分子吡啶部分对称呼吸的光谱峰强度，这可显著加速少量尼古丁的挥发性释放，从而评估不同条件下的尼古丁释放。基于此策略，有可能获得产品的储存或放置条件。本研究结果为阐明各种储存条件下尼古丁的挥发性释放提供了参考，有助于更好地调节口含烟中的尼古丁水平。此外，这种对口含烟产品在不同储存条件下尼古丁挥发性释放的无损评估为进一步研究烟碱类物质对吸烟者健康的影响和戒烟计划开辟了新的视角。
