Quenching effect of cerium oxide nanoparticles on singlet oxygen: validation of the potential for reaction with multiple reactive oxygen species.

Here we studied cerium oxide nanoparticles (nanoceria) as an agent for the future treatment of oxidative damage by validating and evaluating its scavenging activity towards reactive oxygen species (ROS) . Nanoceria has been shown to mimic the activities of superoxide dismutase and catalase, degrading superoxide (O) and hydrogen peroxide (HO). We examined the antioxidative activity of nanoceria, focusing on its ability to quench singlet oxygen (O) in an aqueous solution. Electron paramagnetic resonance (EPR) was used to determine the rates of second-order reactions between nanoceria and three ROS (O, O, and HO) in aqueous solution, and its antioxidative abilities were demonstrated. Nanoceria shows a wide range of ultraviolet-light absorption bands and thus O was produced directly in a nanoceria suspension using high-frequency ultrasound. The quenching or scavenging abilities of nanoceria for O and hypoxanthine-xanthine oxidase reaction-derived O were examined by EPR spin-trapping methods, and the consumption of HO was estimated by the EPR oximetry method. Our results indicated that nanoceria interact not only with two previously reported ROS but also with O. Nanoceria were shown to degrade O and HO, and their ability to quench O may be one mechanism by which they protect against oxidative damage such as inflammation.