Jin Y J, Sun L, Zhao J X, Liu X Y
Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2023 Aug 18;55(4):631-635. doi: 10.19723/j.issn.1671-167X.2023.04.010.
To detect serum IgA isotype of anti-v-raf murine sarcoma viral oncogene homologue B1 (BRAF) antibody levels in the rheumatoid arthritis (RA) patients in order to investigate their clinical significance in RA.
Serum samples were obtained from 61 RA patients, 21 osteoarthritis (OA) patients, 16 systemic lupus erythematosus (SLE) patients, 16 gout patients, 16 Sjögren's syndrome (SS) patients and 22 healthy controls. IgA isotype of anti-BRAF antibody levels in the sera were examined by enzyme-linked immunosorbent assay (ELISA). The associations between IgA isotype of anti-BRAF antibody levels and the clinical features including age, disease duration and laboratory parameters including erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF), disease activity score in 28 joints (DAS28), anti-cyclic citrullinated peptide (anti-CCP) antibody, immunoglobulin and BRAF protein levels in the RA patients were evaluated. Data analyses were performed by using SPSS 19.0 program.
The serum IgA isotype of anti-BRAF antibody levels in the RA patients were significantly higher than in the SLE, gout, OA patients and healthy controls, the value was 0.011, < 0.001, < 0.001 and < 0.001, respectively. The serum IgA isotype of anti-BRAF antibody levels in the SS patients were also significantly higher than in the SLE, gout, OA patients and healthy controls, the value was 0.029, 0.004, 0.001 and < 0.001, respectively. However, there was no difference between the RA and SS patients (=0.762). IgA isotype of anti-BRAF antibody was measurable in the RA patients without RF, anti-CCP antibody or anti-keratin antibody (AKA) antibodies. The levels of IgA isotype of anti-BRAF antibody in the RA patients did not show any correlation with clinical features such as age and disease duration or laboratory parameters including ESR, CRP, RF, DAS28, anti-CCP antibody, immunoglobulin and BRAF protein levels. Compared with the clinical features and laboratory indexes of normal and elevated levels of IgA isotype of anti-BRAF antibody groups in the RA patients, there was no significant differences between the two groups in age, disease duration, ESR, CRP, RF, DAS28, anti-CCP antibody, immunoglobulin or BRAF protein levels.
The elevated level of IgA isotype of anti-BRAF antibody in the RA patients showed that IgA isotype of anti-BRAF antibody might play a role in the pathogenesis of RA. Furthermore, detection of IgA isotype of anti-BRAF antibody in the serum negative RA patients showed that it might be helpful for the diagnosis of the serum negative RA patients.
检测类风湿关节炎（RA）患者血清中抗v-raf鼠肉瘤病毒癌基因同源物B1（BRAF）抗体的IgA同种型水平，以探讨其在RA中的临床意义。
收集61例RA患者、21例骨关节炎（OA）患者、16例系统性红斑狼疮（SLE）患者、16例痛风患者、16例干燥综合征（SS）患者及22例健康对照者的血清样本。采用酶联免疫吸附测定（ELISA）检测血清中抗BRAF抗体的IgA同种型水平。评估RA患者中抗BRAF抗体IgA同种型水平与年龄、病程等临床特征以及红细胞沉降率（ESR）、C反应蛋白（CRP）、类风湿因子（RF）、28个关节疾病活动评分（DAS28）、抗环瓜氨酸肽（抗CCP）抗体、免疫球蛋白及BRAF蛋白水平等实验室指标之间的相关性。使用SPSS 19.0软件进行数据分析。
RA患者血清中抗BRAF抗体的IgA同种型水平显著高于SLE患者、痛风患者、OA患者及健康对照者，P值分别为0.011、<0.001、<0.001及<0.001。SS患者血清中抗BRAF抗体的IgA同种型水平也显著高于SLE患者、痛风患者、OA患者及健康对照者，P值分别为0.029、0.004、0.001及<0.001。然而，RA与SS患者之间无差异（P=0.762）。在无RF、抗CCP抗体或抗角蛋白抗体（AKA）的RA患者中可检测到抗BRAF抗体的IgA同种型。RA患者中抗BRAF抗体的IgA同种型水平与年龄、病程等临床特征或ESR、CRP、RF、DAS28、抗CCP抗体、免疫球蛋白及BRAF蛋白水平等实验室指标均无相关性。比较RA患者中抗BRAF抗体IgA同种型水平正常和升高组的临床特征及实验室指标，两组在年龄、病程、ESR、CRP、RF、DAS28、抗CCP抗体、免疫球蛋白或BRAF蛋白水平方面均无显著差异。
RA患者中抗BRAF抗体的IgA同种型水平升高表明其可能在RA发病机制中起作用。此外，检测血清学阴性RA患者血清中抗BRAF抗体的IgA同种型可能有助于血清学阴性RA患者的诊断。
