Kumar Manishekhar, Banerjee Pradipta, Das Amitava, Singh Kanhaiya, Guith Tanner, Kacar Sedat, Gourishetti Karthik, Sen Chandan K, Roy Sashwati, Khanna Savita
McGowan Institute for Regenerative Medicine, Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Department of Surgery, Indiana Center for Regenerative Medicine and Engineering, Indiana University Health Comprehensive Wound Center, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Adv Wound Care (New Rochelle). 2024 Feb;13(2):70-82. doi: 10.1089/wound.2023.0065. Epub 2023 Sep 13.
Hydrolyzed collagen-based matrices are widely used as wound care dressings. Information on the mechanism of action of such dressings is scanty. The objective of this study was to test the effect of a specific hydrolyzed collagen powder (HCP), which is extensively used for wound care management in the United States. The effects of HCP on resolution of wound inflammation, perfusion, closure, and breaking strength of the repaired skin were studied in an experimental murine model. In early (day 7) inflammatory phase of wound macrophages, HCP treatment boosted phagocytosis and efferocytosis of wound-site macrophages. In these cells, inducible reactive oxygen species were also higher on day (d) 7. HCP treatment potentiated the expression of anti-inflammatory interleukin (IL)-10 cytokine and proangiogenic vascular endothelial growth factor (VEGF) production. Excisional wounds dressed with HCP showed complete closure on day 21, while the control wounds remained open. HCP treatment also demonstrated improved quality of wound healing as marked by the improved breaking strength of the closed wound tissue/repaired skin. These data represent first evidence on the mechanism of action of clinically used HCP. HCP dressing favorably influenced both wound inflammation and vascularization. Improved breaking strength of HCP-treated repaired skin lays the rationale for future studies testing the hypothesis that HCP-treated closed wounds would show fewer recurrences.
基于水解胶原蛋白的基质被广泛用作伤口护理敷料。关于此类敷料作用机制的信息很少。本研究的目的是测试一种在美国广泛用于伤口护理管理的特定水解胶原蛋白粉末（HCP）的效果。在实验性小鼠模型中研究了HCP对伤口炎症消退、灌注、闭合以及修复皮肤的抗张强度的影响。在伤口巨噬细胞的早期（第7天）炎症阶段，HCP处理增强了伤口部位巨噬细胞的吞噬作用和胞葬作用。在这些细胞中，第7天诱导型活性氧也更高。HCP处理增强了抗炎白细胞介素（IL）-10细胞因子的表达和促血管生成的血管内皮生长因子（VEGF）的产生。用HCP包扎的切除伤口在第21天完全闭合，而对照伤口仍未愈合。HCP处理还显示出伤口愈合质量的改善，表现为闭合伤口组织/修复皮肤的抗张强度提高。这些数据代表了临床上使用的HCP作用机制的首个证据。HCP敷料对伤口炎症和血管生成均有有利影响。HCP处理的修复皮肤抗张强度的提高为未来测试HCP处理的闭合伤口复发较少这一假设的研究奠定了理论基础。
