Department of Chemistry, University of Massachusetts Amherst, 710 N. Pleasant Street, Amherst, Massachusetts 01003, United States.
Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, 230 Stockbridge Road, Amherst, Massachusetts 01003, United States.
Anal Chem. 2023 Aug 15;95(32):12177-12183. doi: 10.1021/acs.analchem.3c02551. Epub 2023 Aug 3.
Macrophages are key components of the innate immune system that have essential functions in physiological processes and diseases. The phenotypic plasticity of macrophages allows cells to be polarized into a multidimensional spectrum of phenotypes, broadly classed as pro-inflammatory (M1) and anti-inflammatory (M2) states. Repolarization of M1 to M2 phenotypes alters the immune response to ameliorate autoimmune and inflammation-associated diseases. Detection of this repolarization, however, is challenging to execute in high-throughput applications. In this work, we demonstrate the ability of a single polymer fabricated to provide a six-channel sensor array that can determine macrophage polarization phenotypes. This sensing platform provides a sensitive and high-throughput tool for detecting drug-induced M1-to-M2 repolarization, allowing the identification of new therapeutic leads for inflammatory diseases. The ability of this sensor array to discriminate different M2 subtypes induced by drugs can also improve the efficacy evaluation of anti-inflammatory drugs and avoid adverse effects.
巨噬细胞是先天免疫系统的关键组成部分，在生理过程和疾病中具有重要功能。巨噬细胞的表型可塑性允许细胞被极化到一个多维的表型谱中，大致分为促炎（M1）和抗炎（M2）状态。M1 向 M2 表型的再极化改变了免疫反应，以改善自身免疫和炎症相关疾病。然而，这种再极化的检测在高通量应用中执行具有挑战性。在这项工作中，我们展示了一种由单个聚合物制造的传感器阵列的能力，该传感器阵列可以确定巨噬细胞极化表型。这种传感平台为检测药物诱导的 M1 向 M2 再极化提供了一种敏感和高通量的工具，为炎症性疾病确定了新的治疗靶点。该传感器阵列区分药物诱导的不同 M2 亚型的能力也可以提高抗炎药物的疗效评估，并避免不良反应。
