Department of Mechanical and Industrial Engineering, Qatar university, Qatar; Biomedical Research Center, Qatar University, Qatar.
Dow Institute for Advanced Biological and Animal Research, Dow International Medical College, Dow University of Health Sciences, Qatar.
Biomed Pharmacother. 2023 Sep;165:115156. doi: 10.1016/j.biopha.2023.115156. Epub 2023 Aug 2.
Impaired wound healing is a major healthcare problem in patients with diabetes often resulting in gangrene, microbial infection and amputation of affected limb. The delay or absence in healing process arises from several abnormalities, among them chronic hypoxia is a major concern due to its associated issues such as lack of collagen deposition, epithelization, fibroplasia, angiogenesis, and resistance to infections at the wound site. To address hypoxia, delivery of oxygen at the wound site through oxygen releasing agents have been proven to be effective therapeutics. Several oxygen releasing nanoparticles such as Sodium Percarbonate (SPC), Calcium Peroxide (CPO), Hydrogen Peroxide, Magnesium Peroxide (MPO) have been investigated in wound healing application. However, the uncontrolled/burst release of these nanotherapeutic agents and its accompanied cytotoxicity pose a barrier in expediting the healing process. In this study, a Chitosan-Polyvinyl alcohol (CS-PVA) based hydrogel containing oxygen releasing nanoparticle, calcium peroxide (CPO) was constructed to provide a slow and sustained delivery of oxygen for at least 5 days. In-vitro cell culture studies with this material using fibroblast and endothelial cell line exhibited improved biocompatibility, cell viability and enhanced proliferation in comparison with the control group. Additionally, cell migration study using scratch assay method showed superior cell migration ability of our proposed materials. Furthermore, In vivo study using diabetic rat model showed accelerated wound closure rate compared to untreated control wounds.
伤口愈合受损是糖尿病患者的一个主要医疗保健问题，常导致坏疽、微生物感染和受影响肢体的截肢。愈合过程的延迟或缺失源于几种异常，其中慢性缺氧是一个主要关注点，因为其相关问题如胶原沉积、上皮化、纤维增生、血管生成和对感染的抵抗力缺乏等。为了解决缺氧问题，通过释放氧气的制剂向伤口部位输送氧气已被证明是有效的治疗方法。已经研究了几种释放氧气的纳米颗粒，如过碳酸钠（SPC）、过氧化钙（CPO）、过氧化氢、过氧化镁（MPO），用于伤口愈合应用。然而，这些纳米治疗剂的不受控制/爆发释放及其伴随的细胞毒性在加速愈合过程方面构成了障碍。在这项研究中，构建了一种基于壳聚糖-聚乙烯醇（CS-PVA）的水凝胶，其中含有释放氧气的纳米颗粒过氧化钙（CPO），以提供至少 5 天的缓慢和持续的氧气输送。使用成纤维细胞和内皮细胞系进行的这种材料的体外细胞培养研究表明，与对照组相比，其具有更好的生物相容性、细胞活力和增强的增殖能力。此外，使用划痕试验方法进行的细胞迁移研究表明，我们提出的材料具有优越的细胞迁移能力。此外，使用糖尿病大鼠模型的体内研究表明，与未经治疗的对照伤口相比，伤口闭合率得到了加速。
