Tripathi Satyavrat, Singh Bhisham Narayan, Divakar Singh, Kumar Gaurav, Mallick Sarada Prasanna, Srivastava Pradeep
School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Department of Ageing, School of Life Sciences, Manipal Academy of Higher Education, Manipal University, Manipal, Karnataka 576104, India.
Biomed Mater. 2021 Feb 24;16(2):025021. doi: 10.1088/1748-605X/abd1b8.
Hypoxia and sepsis are key concerns towards modern regenerative medicine. Oxygen generating biomaterials having antibacterial property aims to answer these concerns. Hypoxia promotes reactive oxygen species at the implant site that delays wound healing. Sepsis in wound also contributes to delay in wound healing. Therefore, scaffold with antibacterial property and oxygen-producing capacities have shown ability to promote wound healing. In the present study oxygen releasing, ciprofloxacin loaded collagen chitosan scaffold was fabricated for sustained oxygen delivery. Calcium peroxide (CPO) acted as a chemical oxygen source. Oxygen release pattern exhibited a sustained release of oxygen with uniform deposition of CPO on the scaffold. The drug release study shows a prolonged, continuous, and sustained release of ciprofloxacin. Cell culture studies depict that scaffold has suitable cell attachment and migration properties for fibroblasts. In vivo studies performed in the skin flip model visually shows better wound healing and less necrosis. Histological studies show the maintenance of tissue architecture and the deposition of collagen. The results demonstrate that the proposed CPO coated ciprofloxacin loaded collagen-chitosan scaffold can be a promising candidate for skin tissue engineering.
缺氧和脓毒症是现代再生医学面临的关键问题。具有抗菌性能的产氧生物材料旨在解决这些问题。缺氧会在植入部位促进活性氧的产生，从而延迟伤口愈合。伤口中的脓毒症也会导致伤口愈合延迟。因此，具有抗菌性能和产氧能力的支架已显示出促进伤口愈合的能力。在本研究中，制备了负载环丙沙星的胶原壳聚糖支架以实现持续的氧气输送，该支架具有释放氧气的功能。过氧化钙（CPO）作为化学氧源。氧气释放模式显示出氧气的持续释放，且CPO在支架上均匀沉积。药物释放研究表明环丙沙星具有延长、连续和持续释放的特性。细胞培养研究表明，该支架对成纤维细胞具有合适的细胞附着和迁移特性。在皮肤翻转模型中进行的体内研究直观地显示出更好的伤口愈合和更少的坏死。组织学研究显示组织结构得以维持且有胶原蛋白沉积。结果表明，所提出的CPO包被的负载环丙沙星的胶原-壳聚糖支架有望成为皮肤组织工程的候选材料。
