Ministry of Interior, Public Security, Forensic Evidence Laboratories, Criminal Examinations, Riyadh, Saudi Arabia.
Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, P.O. Box 6830, Riyadh 11452, Saudi Arabia.
Forensic Sci Int. 2023 Sep;350:111684. doi: 10.1016/j.forsciint.2023.111684. Epub 2023 Apr 11.
This study aimed to identify the DNA STR profiles that were obtained from the lips with various lip cosmetics involved in lip pencil, lipsticks and lip gloss for a brand - Makeup Forever and lip balms (Labello brand) - have been popularly used by Saudi women at KSA. The study was involved 35 unrelated participants (healthy female donors) aged between 26 and 32. The swabbing of lip cosmetics was done prior to using them as negative control samples, other sterilized swabs were collected from the used lip cosmetics which contained the lip cells for each participant as a study sample. Moreover, the buccal swabs were firmly collected from the cleaned oral cavities for the same donors as reference samples. The air-drying of the collected swabs was done for ten minutes at room temperature and then stored them at - 20 °C before the DNA analysis. The 7500 Real-Time PCR (qRT-PCR) was quantified the extracted DNA. The amplification of 16 STR loci was done using the AmpFlSTR® Identifiler® PCR amplification kit using the Thermocycler ABI 9700 to amplify the extracted DNA. The Applied Bio-systems 3130™ Genetic Analyzer with Gene Mapper® ID-X Software v3.5 was used to analyze the PCR products. The data for quantifying DNA recorded significant decrease in the concentrations of DNA samples ranged from 0.15 to 0.55 ng/µL in comparison to the reference samples, while DNA was not detected in all the negative control samples. Some STR loci showed considerably high inhibition and low heterozygosity loss in the study samples compared to the reference and negative samples. The possibility of extracting DNA samples from lip cosmetics were used in the present study could be useful and successful in some cases due to the effect of the chemical compositions such as heavy pigments, organic components, and aromatic wax on the STR profiles in the lip cosmetics, especially in the lipsticks, lip glosses and lip pencils.
本研究旨在鉴定从嘴唇上获得的 DNA STR 谱，这些嘴唇上涂有各种唇妆产品，包括品牌 Makeup Forever 的唇彩笔、口红和唇彩，以及沙特女性在沙特阿拉伯常用的唇膏（Labello 品牌）。研究涉及 35 名无关参与者（健康女性供体），年龄在 26 至 32 岁之间。在使用唇妆产品作为阴性对照样本之前，对其进行了拭子采样，从每个参与者使用的含有唇细胞的已用唇妆产品中收集了其他消毒拭子作为研究样本。此外，从同一供体的清洁口腔中牢固地收集了口腔拭子作为参考样本。收集的拭子在室温下风干十分钟，然后在 -20°C 下储存，以便进行 DNA 分析。使用 7500 实时 PCR（qRT-PCR）定量提取的 DNA。使用 AmpFlSTR®Identifiler®PCR 扩增试剂盒和 Thermocycler ABI 9700 扩增提取的 DNA，扩增 16 个 STR 基因座。使用 Applied Bio-systems 3130™遗传分析仪和 Gene Mapper®ID-X 软件 v3.5 分析 PCR 产物。用于量化 DNA 的数据记录显示，与参考样本相比，DNA 浓度从 0.15 到 0.55ng/µL 显著下降，而所有阴性对照样本中均未检测到 DNA。与参考和阴性样本相比，研究样本中的一些 STR 基因座显示出相当高的抑制和低杂合性丢失。由于化学组成的影响，如重色素、有机成分和芳香蜡对唇妆产品中的 STR 谱的影响，本研究中从唇妆产品中提取 DNA 样本的可能性在某些情况下可能是有用且成功的，尤其是在口红、唇彩和唇彩笔中。
