Wang Yidan, Xianyu Yunlei
State Key Laboratory of Fluid Power and Mechatronic Systems, College of Biosystems Engineering and Food Science, Zhejiang University, 310058, Hangzhou, China.
State Key Laboratory of Fluid Power and Mechatronic Systems, College of Biosystems Engineering and Food Science, Zhejiang University, 310058, Hangzhou, China; Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Sir Run Run Shaw Hospital, 310016, Hangzhou, China; Future Food Laboratory, Innovation Center of Yangtze River Delta, Zhejiang University, 314100, Jiaxing, China; Ningbo Research Institute, Zhejiang University, 315100, Ningbo, China.
Biosens Bioelectron. 2023 Oct 1;237:115553. doi: 10.1016/j.bios.2023.115553. Epub 2023 Jul 26.
Dual-mode biosensors have gained great attention due to their excellent detection accuracy provided by the mutual verification of output signals. However, current strategies for dual-mode sensing mainly rely on a signal probe exhibiting dual properties that may encounter unreliability. Herein, we report a dual-mode biosensing strategy by modulating the plasmonic and catalytic activities of nanoparticles through a surface growing approach. Ascorbic acid enables the growing of Au shell on Au@Pt NPs to tune both their peroxidase-like activity and plasmonic signal. Enzyme-catalyzed reactions can generate ascorbic acid to modulate the plasmonic and catalytic activities of nanoparticles. Combined with enzyme-linked immunosorbent assay, it enables dual-mode immunoassays of carbofuran with both a colorimetric readout by a spectrometer down to 0.1 ppb and a naked-eye readout of 5 ppb. This dual-mode biosensing technique advantages in tunable sensitivity and robustness, holding promise as an analytical platform for biomedical diagnosis and food safety.
双模生物传感器因其通过输出信号的相互验证提供的出色检测准确性而备受关注。然而，当前的双模传感策略主要依赖于具有双重性质的信号探针，这可能会遇到不可靠性。在此，我们报告了一种通过表面生长方法调节纳米颗粒的等离子体和催化活性的双模生物传感策略。抗坏血酸能够使金壳在Au@Pt纳米颗粒上生长，从而调节其类过氧化物酶活性和等离子体信号。酶催化反应可以产生抗坏血酸来调节纳米颗粒的等离子体和催化活性。结合酶联免疫吸附测定法，它能够实现对呋喃丹的双模免疫测定，通过光谱仪进行比色读出低至0.1 ppb，肉眼读出为5 ppb。这种双模生物传感技术在灵敏度可调性和稳健性方面具有优势，有望成为生物医学诊断和食品安全的分析平台。
