Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Beijing, 100081, China.
College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
Mikrochim Acta. 2024 Oct 1;191(10):637. doi: 10.1007/s00604-024-06718-1.
Gold-platinum (Au@Pt) nanozymes with high catalytic activity and stability were designed to improve the stability of the enzyme-linked immunosorbent assay (ELISA), and a two-mode signal output was used to enhance the sensitivity and confidence of the assay. This study reports the two-mode signal output based on Au@Pt nanozyme to catalyzed 3,3',5,5'-tetramethylbenzidine (TMB) reaction. Oxidized 3,3',5,5'-tetramethylbenzidine (ox-TMB) has wide absorption spectrum, providing excellent optical density capabilities and fluorescence quenching. The detection limits of imidacloprid were 0.88 μg/L and 1.14 μg/L in colorimetric and fluorescence modes, respectively. Multiple-mode strategy improves detection accuracy, increases the confidence of experimental results, and broadens detection modes. Two modes can meet the requirements of accurate and flexible multi-mode sensing in different application situations.
金铂（Au@Pt）纳米酶具有高催化活性和稳定性，被设计用来提高酶联免疫吸附测定（ELISA）的稳定性，并且使用双模信号输出来增强测定的灵敏度和置信度。本研究报道了基于 Au@Pt 纳米酶的双模信号输出，以催化 3,3',5,5'-四甲基联苯胺（TMB）反应。氧化的 3,3',5,5'-四甲基联苯胺（ox-TMB）具有宽的吸收光谱，提供了极好的光密度能力和荧光猝灭。在比色和荧光模式下，吡虫啉的检测限分别为 0.88 μg/L 和 1.14 μg/L。多模式策略提高了检测精度，增加了实验结果的置信度，并拓宽了检测模式。两种模式可以满足不同应用情况下准确和灵活的多模式传感的要求。
