Zhang Han, Peng Xiang, Song Hao, Tan Yongfeng, Xu Jianglian, Li Qunfang, Gao Zhuangqiang
School of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi 445000, China.
Marshall Laboratory of Biomedical Engineering, Shenzhen Key Laboratory for Nano-Biosensing Technology, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen 518060, China.
Biosensors (Basel). 2025 Apr 9;15(4):239. doi: 10.3390/bios15040239.
Interleukin-12 (IL-12), a crucial biomarker for immune and inflammatory responses, plays a pivotal role in diagnosing and managing diverse pathological conditions. Although colorimetric enzyme-linked immunosorbent assays (CELISAs) have been extensively employed to detect IL-12 in biological samples, their sensitivity is inherently limited by the catalytic efficiency of enzyme labels, presenting substantial challenges in achieving ultrasensitive detection and enabling pre-symptomatic diagnosis of diseases. In this study, we address this limitation by developing a novel peroxidase nanozyme, featuring ultrathin Pt skins consisting of only ~4 atomic layers, coated on Au nanoparticles (denoted as Au@PtNPs). These Au@PtNPs exhibit remarkable catalytic performance, achieving a ~1063-fold enhancement in peroxidase-like activity compared to horseradish peroxidase (HRP), while minimizing Pt consumption, thereby improving Pt utilization efficiency and reducing costs. This advancement facilitates the construction of an ultrasensitive CELISA capable of detecting IL-12 at femtomolar concentrations. Using Au@PtNPs as the signal labels, the developed CELISA demonstrates a quantitative detection range from 0.1 to 100 pg mL, with a limit of detection (LOD) as low as 0.084 pg mL (1.1 fM), offering ~10 times greater sensitivity than the HRP-based CELISA. This study highlights the potential of Au@PtNP nanozymes as advanced signal labels, opening new avenues for next-generation ultrasensitive bioassays.
白细胞介素-12（IL-12）是免疫和炎症反应的关键生物标志物，在多种病理状况的诊断和管理中发挥着关键作用。尽管比色酶联免疫吸附测定法（CELISAs）已被广泛用于检测生物样品中的IL-12，但其灵敏度本质上受到酶标记催化效率的限制，在实现超灵敏检测和疾病症状前诊断方面面临重大挑战。在本研究中，我们通过开发一种新型过氧化物酶纳米酶来解决这一限制，该纳米酶以仅由约4个原子层组成的超薄铂皮包裹在金纳米颗粒上（记为Au@PtNPs）。这些Au@PtNPs表现出卓越的催化性能，与辣根过氧化物酶（HRP）相比，其类过氧化物酶活性提高了约1063倍，同时将铂的消耗量降至最低，从而提高了铂的利用效率并降低了成本。这一进展有助于构建一种能够检测飞摩尔浓度IL-12的超灵敏CELISA。使用Au@PtNPs作为信号标记，所开发的CELISA的定量检测范围为0.1至100 pg/mL，检测限（LOD）低至0.084 pg/mL（1.1 fM），灵敏度比基于HRP的CELISA高约10倍。本研究突出了Au@PtNP纳米酶作为先进信号标记的潜力，为下一代超灵敏生物测定开辟了新途径。
